Raspberry Viruses in the Czech Republic, with Identification of a Novel Virus: Raspberry Virus A

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F25271121%3A_____%2F25%3AN0000110" target="_blank" >RIV/25271121:_____/25:N0000110 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/60077344:_____/25:00643150

Výsledek na webu

<a href="https://doi.org/10.3390/v17121597" target="_blank" >https://doi.org/10.3390/v17121597</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.3390/v17121597" target="_blank" >10.3390/v17121597</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Raspberry Viruses in the Czech Republic, with Identification of a Novel Virus: Raspberry Virus A

Popis výsledku v původním jazyce

Although global raspberries production has grown in the past decade, it remains threatened by plant viruses. This study surveyed raspberry viruses and associated arthropods in the Czech Republic between 2021 and 2022 across five regions. A total of 257 plant and 151 arthropod samples were tested using RT-(q)PCR for 12 viruses listed in the EPPO Certification scheme, plus raspberry leaf blotch virus (RLBV) and a novel virus, tentatively named raspberry-associated virus A (RaVA). Raspberry bushy dwarf virus (RBDV) was most prevalent (51.8%), followed by black raspberry necrosis virus (BRNV, 42.0%) and raspberry leaf mottle virus (RLMV, 28.4%). Four viruses—arabis mosaic virus, apple mosaic virus, strawberry latent ringspot virus, raspberry ringspot virus—were not detected. RBDV was also identified in Sambucus nigra, a new host, while mixed RLBV and RaVA infection was found in wild Rubus occidentalis. RLBV was experimentally transmitted to Nicotiana occidentalis 37B in the presence of Phyllocoptes gracilis. Seven of 39 arthropod species carried viruses, but only two—Amphorophora rubi idaei and Aphis idaei—are known vectors. PCR amplicons from 92 isolates were sequenced, revealing high variability in several viruses. These findings offer new insights but highlight the need for continued monitoring and research.

Název v anglickém jazyce

Raspberry Viruses in the Czech Republic, with Identification of a Novel Virus: Raspberry Virus A

Popis výsledku anglicky

Although global raspberries production has grown in the past decade, it remains threatened by plant viruses. This study surveyed raspberry viruses and associated arthropods in the Czech Republic between 2021 and 2022 across five regions. A total of 257 plant and 151 arthropod samples were tested using RT-(q)PCR for 12 viruses listed in the EPPO Certification scheme, plus raspberry leaf blotch virus (RLBV) and a novel virus, tentatively named raspberry-associated virus A (RaVA). Raspberry bushy dwarf virus (RBDV) was most prevalent (51.8%), followed by black raspberry necrosis virus (BRNV, 42.0%) and raspberry leaf mottle virus (RLMV, 28.4%). Four viruses—arabis mosaic virus, apple mosaic virus, strawberry latent ringspot virus, raspberry ringspot virus—were not detected. RBDV was also identified in Sambucus nigra, a new host, while mixed RLBV and RaVA infection was found in wild Rubus occidentalis. RLBV was experimentally transmitted to Nicotiana occidentalis 37B in the presence of Phyllocoptes gracilis. Seven of 39 arthropod species carried viruses, but only two—Amphorophora rubi idaei and Aphis idaei—are known vectors. PCR amplicons from 92 isolates were sequenced, revealing high variability in several viruses. These findings offer new insights but highlight the need for continued monitoring and research.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10607 - Virology

Projekt

<a href="/cs/project/TO01000295" target="_blank" >TO01000295: Zdravé ovoce v měnících se klimatických podmínkách: vývoj nových biotechnologických postupů diagnostiky virů, studium vektorů, ozdravování a bezpečného uchovávání jahodníku a maliníku</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2025

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Viruses

ISSN

1999-4915

e-ISSN

—

Svazek periodika

17

Číslo periodika v rámci svazku

12

Stát vydavatele periodika

CH - Švýcarská konfederace

Počet stran výsledku

27

Strana od-do

1597

Kód UT WoS článku

001647547500001

EID výsledku v databázi Scopus

2-s2.0-105025751239