Effect of selected Indonesian plants on Giardia intestinalis in an experimental in vitro model

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60077344%3A_____%2F25%3A00616973" target="_blank" >RIV/60077344:_____/25:00616973 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/61389030:_____/25:00616973 RIV/00216224:14310/25:00144259 RIV/61989592:15310/25:73634833

Výsledek na webu

<a href="https://doi.org/10.1186/s12906-025-04755-8" target="_blank" >https://doi.org/10.1186/s12906-025-04755-8</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1186/s12906-025-04755-8" target="_blank" >10.1186/s12906-025-04755-8</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Effect of selected Indonesian plants on Giardia intestinalis in an experimental in vitro model

Popis výsledku v původním jazyce

Background nThis study evaluated in vitro antigiardial activity in four Indonesian plants (Archidendron fagifolium, Diospyros sumatrana, Piper betle and Shorea sumatrana) extracted in methanol, methanol-tetrahydrofuran, and water. These plants exhibiting promising anti-parasitic activity were selected on the basis of collected behavioral data and their ability to decrease parasite load in Sumatran orangutans. Extracts of Arabidopsis thaliana, a plant routinely used as a laboratory model in research, were used as a negative control.nMethodsnPlant extracts of different concentrations (400, 100, 25 and 6.25 mu g/ml) and metronidazole (100 mu g/ml), a standard giardicidal drug, were incubated with 25,000 trophozoites per milliliter of growth medium in 12-well tissue culture plates under anaerobic conditions for 72 h. Cultures were counted in a hemocytometer using a light microscope and then statistically evaluated. The cytotoxicity of the extracts was determined by the MTT Cell Viability Assay.nResultsnWe recorded a statistically-significant decrease in giardia trophozoites in two extracts: the methanolic extract of A. fagifolium (Kruskal-Wallis: p = 0.013) and the aqueous extract of P. betle (Kruskal-Wallis: p = 0.002). Effective concentrations of 400 mu g/ml and 100 mu g/ml, respectively, were revealed. Cytotoxicity was not demonstrated.nConclusionsnThe results of our study show that extracts of A. fagifolium and P. betle have potential as an alternative treatment of G. intestinalis infection. This is the first scientific proof of the biological activity of A. fagifolium, confirming the assumption that orangutans use this plant (as well as P. betle) for self-medication.

Název v anglickém jazyce

Effect of selected Indonesian plants on Giardia intestinalis in an experimental in vitro model

Popis výsledku anglicky

Background nThis study evaluated in vitro antigiardial activity in four Indonesian plants (Archidendron fagifolium, Diospyros sumatrana, Piper betle and Shorea sumatrana) extracted in methanol, methanol-tetrahydrofuran, and water. These plants exhibiting promising anti-parasitic activity were selected on the basis of collected behavioral data and their ability to decrease parasite load in Sumatran orangutans. Extracts of Arabidopsis thaliana, a plant routinely used as a laboratory model in research, were used as a negative control.nMethodsnPlant extracts of different concentrations (400, 100, 25 and 6.25 mu g/ml) and metronidazole (100 mu g/ml), a standard giardicidal drug, were incubated with 25,000 trophozoites per milliliter of growth medium in 12-well tissue culture plates under anaerobic conditions for 72 h. Cultures were counted in a hemocytometer using a light microscope and then statistically evaluated. The cytotoxicity of the extracts was determined by the MTT Cell Viability Assay.nResultsnWe recorded a statistically-significant decrease in giardia trophozoites in two extracts: the methanolic extract of A. fagifolium (Kruskal-Wallis: p = 0.013) and the aqueous extract of P. betle (Kruskal-Wallis: p = 0.002). Effective concentrations of 400 mu g/ml and 100 mu g/ml, respectively, were revealed. Cytotoxicity was not demonstrated.nConclusionsnThe results of our study show that extracts of A. fagifolium and P. betle have potential as an alternative treatment of G. intestinalis infection. This is the first scientific proof of the biological activity of A. fagifolium, confirming the assumption that orangutans use this plant (as well as P. betle) for self-medication.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

30107 - Medicinal chemistry

Projekt

<a href="/cs/project/GA23-06571S" target="_blank" >GA23-06571S: Co nás mohou naučit orangutani: protiamébová a protigiardiová aktivita rostlin používaných orangutany k možné sebemedikaci</a><br>

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2025

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

BMC Complementary Medicine and Therapies

ISSN

2662-7671

e-ISSN

2662-7671

Svazek periodika

25

Číslo periodika v rámci svazku

1

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

8

Strana od-do

22

Kód UT WoS článku

001406050800001

EID výsledku v databázi Scopus

2-s2.0-85216718146