Biogenesis of Inner Mitochondrial Membrane Proteins in Trypanosoma brucei
Identifikátory výsledku
Kód výsledku v IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60077344%3A_____%2F25%3A00642777" target="_blank" >RIV/60077344:_____/25:00642777 - isvavai.cz</a>
Výsledek na webu
<a href="https://drive.google.com/file/d/1VBqtyqlV1Jc9A3c89jJNFkVdGGSv985u/view" target="_blank" >https://drive.google.com/file/d/1VBqtyqlV1Jc9A3c89jJNFkVdGGSv985u/view</a>
DOI - Digital Object Identifier
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Alternativní jazyky
Jazyk výsledku
angličtina
Název v původním jazyce
Biogenesis of Inner Mitochondrial Membrane Proteins in Trypanosoma brucei
Popis výsledku v původním jazyce
The mitochondrial insertase Oxa1 is essential for the insertion of nuclear- and mitochondrial-encoded transmembrane proteins and the biogenesis of respiratory chain (RC) complexes at the inner mitochondrial membrane (IMM). Trypanosoma brucei, a parasitic protist with a single mitochondrion, encodes three putative TbOxa1 paralogs. Whether these paralogs have substrate specificity or specialized roles in IMM biogenesis remains unclear. Using CRISPR/Cas9-mediated ablation in procyclic form (PF) T. brucei, we observed reduced cell viability, accumulation of RC complex subunit precursors, and impaired RC complex integrity upon loss of individual TbOxa1s. Submitochondrial fractionation combined with label-free quantitative mass spectrometry revealed distinct proteomic alterations across mitochondrial subcompartments, indicating both shared and specific roles of TbOxa1 paralogs in IMM protein biogenesis, particularly affecting RC complex components.
Název v anglickém jazyce
Biogenesis of Inner Mitochondrial Membrane Proteins in Trypanosoma brucei
Popis výsledku anglicky
The mitochondrial insertase Oxa1 is essential for the insertion of nuclear- and mitochondrial-encoded transmembrane proteins and the biogenesis of respiratory chain (RC) complexes at the inner mitochondrial membrane (IMM). Trypanosoma brucei, a parasitic protist with a single mitochondrion, encodes three putative TbOxa1 paralogs. Whether these paralogs have substrate specificity or specialized roles in IMM biogenesis remains unclear. Using CRISPR/Cas9-mediated ablation in procyclic form (PF) T. brucei, we observed reduced cell viability, accumulation of RC complex subunit precursors, and impaired RC complex integrity upon loss of individual TbOxa1s. Submitochondrial fractionation combined with label-free quantitative mass spectrometry revealed distinct proteomic alterations across mitochondrial subcompartments, indicating both shared and specific roles of TbOxa1 paralogs in IMM protein biogenesis, particularly affecting RC complex components.
Klasifikace
Druh
O - Ostatní výsledky
CEP obor
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OECD FORD obor
10608 - Biochemistry and molecular biology
Návaznosti výsledku
Projekt
<a href="/cs/project/EH22_008%2F0004575" target="_blank" >EH22_008/0004575: RNA pro terapii</a><br>
Návaznosti
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Ostatní
Rok uplatnění
2025
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů