Chromosome-level genome assembly of trypanosomatid parasite Lotmaria passim links chromosome duplication and divergence with infection of honey bees

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60077344%3A_____%2F25%3A00642912" target="_blank" >RIV/60077344:_____/25:00642912 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/61988987:17310/25:A2603CW4 RIV/60076658:12310/25:43910242

Výsledek na webu

<a href="https://doi.org/10.1186/s12864-025-12082-y" target="_blank" >https://doi.org/10.1186/s12864-025-12082-y</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1186/s12864-025-12082-y" target="_blank" >10.1186/s12864-025-12082-y</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Chromosome-level genome assembly of trypanosomatid parasite Lotmaria passim links chromosome duplication and divergence with infection of honey bees

Popis výsledku v původním jazyce

BackgroundThe protist family Trypanosomatidae includes parasites of insects, vertebrates, plants, and even other unicellular eukaryotes. The genomes of these species harbor clues to the evolution of parasitism, adaptation to new hosts, and infection of mammals. We present an analysis of a chromosome-level genome assembly of Lotmaria passim, the most prevalent known trypanosomatid of honey bees, linking genome sequence and organization to gene expression and infection of bees.ResultsThe genome showed a high degree of synteny with assemblies of other trypanosomatids and especially to the closely related Leptomonas pyrrhocoris. It included four copies of chromosomes that shared ancestry with the tetrasomic Leishmania Chromosome 31 and are consistently supernumerary throughout Trypanosomatidae. However, these chromosomes showed lower similarity to L. passim relatives than did the genome overall, with sufficient variation across haplotypes to distinguish two separate disomic chromosomes. Transcriptomic analyses showed that these chromosomes are enriched in genes upregulated during bee infection, and each include five paralogs of the GP63 gene implicated in infection of both insects and mammals. Patterns of expression in bees suggested decreased protein synthesis, a shift from carbohydrate- to amino acid-based metabolism, and reduced cell motility in bee guts versus cell culture. In contrast, genes involved in cell adhesion were upregulated, consistent with the importance of attachment to insect tissue in this species and the family overall.ConclusionsOur analysis links differentiation of a conserved supernumerary chromosome with infection of bees, parallel to this chromosome's role in Leishmania infection of mammals and linking chromosome-level changes with adaptation to new hosts.

Název v anglickém jazyce

Chromosome-level genome assembly of trypanosomatid parasite Lotmaria passim links chromosome duplication and divergence with infection of honey bees

Popis výsledku anglicky

BackgroundThe protist family Trypanosomatidae includes parasites of insects, vertebrates, plants, and even other unicellular eukaryotes. The genomes of these species harbor clues to the evolution of parasitism, adaptation to new hosts, and infection of mammals. We present an analysis of a chromosome-level genome assembly of Lotmaria passim, the most prevalent known trypanosomatid of honey bees, linking genome sequence and organization to gene expression and infection of bees.ResultsThe genome showed a high degree of synteny with assemblies of other trypanosomatids and especially to the closely related Leptomonas pyrrhocoris. It included four copies of chromosomes that shared ancestry with the tetrasomic Leishmania Chromosome 31 and are consistently supernumerary throughout Trypanosomatidae. However, these chromosomes showed lower similarity to L. passim relatives than did the genome overall, with sufficient variation across haplotypes to distinguish two separate disomic chromosomes. Transcriptomic analyses showed that these chromosomes are enriched in genes upregulated during bee infection, and each include five paralogs of the GP63 gene implicated in infection of both insects and mammals. Patterns of expression in bees suggested decreased protein synthesis, a shift from carbohydrate- to amino acid-based metabolism, and reduced cell motility in bee guts versus cell culture. In contrast, genes involved in cell adhesion were upregulated, consistent with the importance of attachment to insect tissue in this species and the family overall.ConclusionsOur analysis links differentiation of a conserved supernumerary chromosome with infection of bees, parallel to this chromosome's role in Leishmania infection of mammals and linking chromosome-level changes with adaptation to new hosts.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10602 - Biology (theoretical, mathematical, thermal, cryobiology, biological rhythm), Evolutionary biology

Projekt

<a href="/cs/project/GA23-07695S" target="_blank" >GA23-07695S: Zkoumání evoluce genomu u eukaryot: poučení z nemodelových euglenozoí</a><br>

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2025

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

BMC Genomics

ISSN

1471-2164

e-ISSN

1471-2164

Svazek periodika

26

Číslo periodika v rámci svazku

1

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

19

Strana od-do

1083

Kód UT WoS článku

001628398200001

EID výsledku v databázi Scopus

2-s2.0-105023452377