Blastocrithidia—A Genetic Alien from the Planet Earth

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60077344%3A_____%2F25%3A00642913" target="_blank" >RIV/60077344:_____/25:00642913 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/61388971:_____/25:00642913 RIV/61988987:17310/25:A2603CYW RIV/60076658:12310/25:43910243

Výsledek na webu

<a href="https://doi.org/10.1101/cshperspect.a041868" target="_blank" >https://doi.org/10.1101/cshperspect.a041868</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1101/cshperspect.a041868" target="_blank" >10.1101/cshperspect.a041868</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Blastocrithidia—A Genetic Alien from the Planet Earth

Popis výsledku v původním jazyce

The standard genetic code, which applies almost without exception, is the key to our understanding of molecular biological processes. Although it is close to impossible to imagine that sparse code changes occur naturally given proteomic constraints, specific cases of codon usage alterations have been documented, mostly in unicellular eukaryotes. Here, we summarize what we have learned about Blastocrithidia, a little-known parasitic flagellate with all three stop codons reassigned to sense codons, which uses UAA as the only universal stop codon. We first describe its origin, life cycle, morphology, cultivation, and transformation, the combination of which predisposes it to become the first tractable eukaryote with a noncanonical genetic code. Next, we present our across-the-genome analysis revealing uneven distribution of in-frame stops and discuss the features distinguishing in-frame and genuine stop codons that allow for so-called position-specific termination. Finally, given what is known about stop codon readthrough by near-cognate transfer RNAs (tRNAs) and the fidelity of stop codon recognition by eukaryotic release factor 1 (eRF1), we propose a model illuminating how unique properties of Blastocrithidia tRNAs, combined with specific alterations of its eRF1, enable this massive deviation from the standard genetic code.

Název v anglickém jazyce

Blastocrithidia—A Genetic Alien from the Planet Earth

Popis výsledku anglicky

The standard genetic code, which applies almost without exception, is the key to our understanding of molecular biological processes. Although it is close to impossible to imagine that sparse code changes occur naturally given proteomic constraints, specific cases of codon usage alterations have been documented, mostly in unicellular eukaryotes. Here, we summarize what we have learned about Blastocrithidia, a little-known parasitic flagellate with all three stop codons reassigned to sense codons, which uses UAA as the only universal stop codon. We first describe its origin, life cycle, morphology, cultivation, and transformation, the combination of which predisposes it to become the first tractable eukaryote with a noncanonical genetic code. Next, we present our across-the-genome analysis revealing uneven distribution of in-frame stops and discuss the features distinguishing in-frame and genuine stop codons that allow for so-called position-specific termination. Finally, given what is known about stop codon readthrough by near-cognate transfer RNAs (tRNAs) and the fidelity of stop codon recognition by eukaryotic release factor 1 (eRF1), we propose a model illuminating how unique properties of Blastocrithidia tRNAs, combined with specific alterations of its eRF1, enable this massive deviation from the standard genetic code.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10608 - Biochemistry and molecular biology

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2025

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Cold Spring Harbor Perspectives in Biology

ISSN

1943-0264

e-ISSN

1943-0264

Svazek periodika

17

Číslo periodika v rámci svazku

12

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

21

Strana od-do

a041868

Kód UT WoS článku

001627580400001

EID výsledku v databázi Scopus

2-s2.0-105023545702