Assessing allergenic potential, exposure and risk of Anisakis spp.

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60077344%3A_____%2F25%3A00643793" target="_blank" >RIV/60077344:_____/25:00643793 - isvavai.cz</a>

Výsledek na webu

<a href="https://doi.org/10.2903/j.efsa.2025.e231106" target="_blank" >https://doi.org/10.2903/j.efsa.2025.e231106</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.2903/j.efsa.2025.e231106" target="_blank" >10.2903/j.efsa.2025.e231106</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Assessing allergenic potential, exposure and risk of Anisakis spp.

Popis výsledku v původním jazyce

Parasites in seafood are a recognised food safety concern worldwide. Anisakiasis is a human disease caused by ingestion of larvae of parasitic nematodes of the genus Anisakis, typically present in raw or undercooked seafood. It is considered a significant emerging foodborne disease and was recently ranked among the top 10 of 24 parasitic foodborne infections in Europe, with a rapidly increasing potential to cause illness. Many clinical cases are often misdiagnosed as other gastrointestinal disorders, leading to misinterpretation of data and likely a substantial underestimation of their true prevalence. The issue is further complicated by a rising number of asymptomatic individuals who develop sensitivity to thermostable Anisakis antigens. These otherwise healthy individuals often show elevated anti-Anisakis IgE antibody levels and may have recovered from a mild or undiagnosed gastric anisakiasis without showing allergic symptoms. Because these cases lack obvious clinical signs, this 'silent' form of anisakiasis could affect more than 12% of certain populations and is typically identified only through sero-epidemiological research. Earlier detection approaches, including DNA-based methods such as real-time PCR, demonstrated the potential to identify Anisakis as an allergy-causing agens in marketed seafood. However, these methods had not been validated, and the results, therefore, required cautious interpretation. Here, we present the first validated real-time PCR method designed to improve the detection of Anisakis as an allergy-causing agens and to support more accurate monitoring within the EU seafood market. Additional work to express two known allergic proteins (Ani s 1 and Ani s 7) for immunological testing of hypersensitised patients can open venues for a future complete risk assessment of anisakiasis.

Název v anglickém jazyce

Assessing allergenic potential, exposure and risk of Anisakis spp.

Popis výsledku anglicky

Parasites in seafood are a recognised food safety concern worldwide. Anisakiasis is a human disease caused by ingestion of larvae of parasitic nematodes of the genus Anisakis, typically present in raw or undercooked seafood. It is considered a significant emerging foodborne disease and was recently ranked among the top 10 of 24 parasitic foodborne infections in Europe, with a rapidly increasing potential to cause illness. Many clinical cases are often misdiagnosed as other gastrointestinal disorders, leading to misinterpretation of data and likely a substantial underestimation of their true prevalence. The issue is further complicated by a rising number of asymptomatic individuals who develop sensitivity to thermostable Anisakis antigens. These otherwise healthy individuals often show elevated anti-Anisakis IgE antibody levels and may have recovered from a mild or undiagnosed gastric anisakiasis without showing allergic symptoms. Because these cases lack obvious clinical signs, this 'silent' form of anisakiasis could affect more than 12% of certain populations and is typically identified only through sero-epidemiological research. Earlier detection approaches, including DNA-based methods such as real-time PCR, demonstrated the potential to identify Anisakis as an allergy-causing agens in marketed seafood. However, these methods had not been validated, and the results, therefore, required cautious interpretation. Here, we present the first validated real-time PCR method designed to improve the detection of Anisakis as an allergy-causing agens and to support more accurate monitoring within the EU seafood market. Additional work to express two known allergic proteins (Ani s 1 and Ani s 7) for immunological testing of hypersensitised patients can open venues for a future complete risk assessment of anisakiasis.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

30310 - Parasitology

Projekt

—

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2025

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

EFSA Journal

ISSN

1831-4732

e-ISSN

1831-4732

Svazek periodika

23

Číslo periodika v rámci svazku

DEC

Stát vydavatele periodika

IT - Italská republika

Počet stran výsledku

9

Strana od-do

e231106

Kód UT WoS článku

001638611100001

EID výsledku v databázi Scopus

2-s2.0-105024873954