Splice site diversity and abundance of noncanonical introns in diplonemids (Diplonemea, Euglenozoa)

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60077344%3A_____%2F25%3A00644363" target="_blank" >RIV/60077344:_____/25:00644363 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/68378050:_____/25:00644363 RIV/61988987:17310/25:A2603CWF RIV/60076658:12310/25:43910289

Výsledek na webu

<a href="https://rnajournal.cshlp.org/content/31/12/1826.full" target="_blank" >https://rnajournal.cshlp.org/content/31/12/1826.full</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1261/rna.080641.125" target="_blank" >10.1261/rna.080641.125</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Splice site diversity and abundance of noncanonical introns in diplonemids (Diplonemea, Euglenozoa)

Popis výsledku v původním jazyce

Noncoding introns are a unifying feature of protein-coding genes in virtually all extant eukaryotes, with most lineages following the canonical intron structure. However, euglenozoans, unicellular flagellates that include free-living euglenids, human pathogenic kinetoplastids, and highly diverse and abundant marine diplonemids, are a notable exception. Euglenozoan genomes range from extremely intron-poor kinetoplastids to euglenid genomes containing both canonical and noncanonical introns. Here, we present a comprehensive analysis of splice sites and spliceosomal components in six species of understudied diplonemids. All diplonemids examined contain a nearly complete set of spliceosomal snRNP components, indicating the presence of a functional U2-type spliceosome. However, the majority of introns in the hemistasiid diplonemids Artemidia motanka and Namystynia karyoxenos are noncanonical and lack conserved GT-AG terminal dinucleotides typical for U2-type introns. These noncanonical introns are capable of extensive base-pairing, which brings intron ends into close proximity. Thus, while the splicing apparatus is conserved in diplonemids, the splice sites are highly variable among individual species.

Název v anglickém jazyce

Splice site diversity and abundance of noncanonical introns in diplonemids (Diplonemea, Euglenozoa)

Popis výsledku anglicky

Noncoding introns are a unifying feature of protein-coding genes in virtually all extant eukaryotes, with most lineages following the canonical intron structure. However, euglenozoans, unicellular flagellates that include free-living euglenids, human pathogenic kinetoplastids, and highly diverse and abundant marine diplonemids, are a notable exception. Euglenozoan genomes range from extremely intron-poor kinetoplastids to euglenid genomes containing both canonical and noncanonical introns. Here, we present a comprehensive analysis of splice sites and spliceosomal components in six species of understudied diplonemids. All diplonemids examined contain a nearly complete set of spliceosomal snRNP components, indicating the presence of a functional U2-type spliceosome. However, the majority of introns in the hemistasiid diplonemids Artemidia motanka and Namystynia karyoxenos are noncanonical and lack conserved GT-AG terminal dinucleotides typical for U2-type introns. These noncanonical introns are capable of extensive base-pairing, which brings intron ends into close proximity. Thus, while the splicing apparatus is conserved in diplonemids, the splice sites are highly variable among individual species.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

30310 - Parasitology

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2025

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

RNA

ISSN

1355-8382

e-ISSN

1469-9001

Svazek periodika

31

Číslo periodika v rámci svazku

12

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

16

Strana od-do

1826-1840

Kód UT WoS článku

001616905800001

EID výsledku v databázi Scopus

2-s2.0-105022305062