Cultivation, genomics, and giant viruses of a ubiquitous and heterotrophic freshwater cryptomonad

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60077344%3A_____%2F25%3A00647074" target="_blank" >RIV/60077344:_____/25:00647074 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/60076658:12310/25:43910593

Výsledek na webu

<a href="https://doi.org/10.1093/ismejo/wraf271" target="_blank" >https://doi.org/10.1093/ismejo/wraf271</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1093/ismejo/wraf271" target="_blank" >10.1093/ismejo/wraf271</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Cultivation, genomics, and giant viruses of a ubiquitous and heterotrophic freshwater cryptomonad

Popis výsledku v původním jazyce

Heterotrophic nanoflagellates are the chief agents of bacterivory in the aquatic microbial loop but remain underrepresented in culture collections and in genomic databases. We isolated and characterized a representative of the previously uncultured freshwater Cryptomonad Group 1 (CRY1a) lineage using a genome-streamlined, ultra-small and abundant microbe Planktophila versatilis as a prey and Catalyzed Reporter Deposition-Fluorescence in situ Hybridization (CARD-FISH) probe-based screening. This isolate, Tyrannomonas regina, is one of the most dominant ubiquitous heterotrophic cryptomonads in freshwaters. It is a small heterotrophic nanoflagellate (ca. 3-5 mu m) and has the smallest genome of any cryptomonad sequenced thus far. The compact genome (ca. 69 Mb) revealed no traces of a photosynthetic lifestyle, consistent with its phylogenomic placement as a sister clade to cryptophytes that are characterized by the acquisition of a red-algal symbiont. Moreover, in comparison to its photosynthetic counterparts, its genome presents substantially lower repeat content and endogenous viral elements. Genomes of two giant viruses, Tyrannovirus reginensis GV1 and GV2, were also recovered from the same culture and represent a viral genus that has been described so far solely by metagenome-recovered genomes. Collectively, these findings provide insights into genomic ancestry and evolution, widespread ecological impact, and interactions of an elusive protist lineage and illustrate the advantages of culture-centric approaches towards unfolding complex tapestries of life in the microbial world.

Název v anglickém jazyce

Cultivation, genomics, and giant viruses of a ubiquitous and heterotrophic freshwater cryptomonad

Popis výsledku anglicky

Heterotrophic nanoflagellates are the chief agents of bacterivory in the aquatic microbial loop but remain underrepresented in culture collections and in genomic databases. We isolated and characterized a representative of the previously uncultured freshwater Cryptomonad Group 1 (CRY1a) lineage using a genome-streamlined, ultra-small and abundant microbe Planktophila versatilis as a prey and Catalyzed Reporter Deposition-Fluorescence in situ Hybridization (CARD-FISH) probe-based screening. This isolate, Tyrannomonas regina, is one of the most dominant ubiquitous heterotrophic cryptomonads in freshwaters. It is a small heterotrophic nanoflagellate (ca. 3-5 mu m) and has the smallest genome of any cryptomonad sequenced thus far. The compact genome (ca. 69 Mb) revealed no traces of a photosynthetic lifestyle, consistent with its phylogenomic placement as a sister clade to cryptophytes that are characterized by the acquisition of a red-algal symbiont. Moreover, in comparison to its photosynthetic counterparts, its genome presents substantially lower repeat content and endogenous viral elements. Genomes of two giant viruses, Tyrannovirus reginensis GV1 and GV2, were also recovered from the same culture and represent a viral genus that has been described so far solely by metagenome-recovered genomes. Collectively, these findings provide insights into genomic ancestry and evolution, widespread ecological impact, and interactions of an elusive protist lineage and illustrate the advantages of culture-centric approaches towards unfolding complex tapestries of life in the microbial world.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10617 - Marine biology, freshwater biology, limnology

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2025

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

The ISME Journal

ISSN

1751-7362

e-ISSN

1751-7370

Svazek periodika

19

Číslo periodika v rámci svazku

1

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

14

Strana od-do

wraf271

Kód UT WoS článku

001649991100001

EID výsledku v databázi Scopus

2-s2.0-105026364181