Cytotoxický efekt extraktů půdních sinic k liniím savčích buněk YAC-1 A WEHI

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60077379%3A_____%2F05%3A00029096" target="_blank" >RIV/60077379:_____/05:00029096 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Cytotoxic effect of soil cyanobacterial extracts to mammal cell lines YAC-1 and WEHI

Popis výsledku v původním jazyce

The cytotoxic effects of ten methanol extracts obtained from soil filamentous cyanobacteria (Anabaena, Calothrix, Nodularia, Cylindrospermum, Tolypothrix, Trichormus) were studied. Two different mammal cell lines (YAC-1, WEHI) were selected for cytotoxicity testing using the MTT test and flow-cytometry (FC). For comparison, the brine shrimp assay (using Artemia as a testing organism) was performed. The composition of extracts was studied using HPLC-MS. Both MTT and FC found the cytotoxic effect in 6 of10 tested cyanobacterial extracts, but these results did not correlate with those obtained by brine shrimp assay. In Anabaena torulosa,Cylindrospermum sp. the most severe damage was recorded by both methods. In cells treated by the extract of A. torulosa, 100% inhibition was found already after 60-minute exposure. Induction of necrosis was revealed by FC, since all tested cells were marked by propidium iodide indicating disruption of the cellular membrane.

Název v anglickém jazyce

Cytotoxic effect of soil cyanobacterial extracts to mammal cell lines YAC-1 and WEHI

Popis výsledku anglicky

The cytotoxic effects of ten methanol extracts obtained from soil filamentous cyanobacteria (Anabaena, Calothrix, Nodularia, Cylindrospermum, Tolypothrix, Trichormus) were studied. Two different mammal cell lines (YAC-1, WEHI) were selected for cytotoxicity testing using the MTT test and flow-cytometry (FC). For comparison, the brine shrimp assay (using Artemia as a testing organism) was performed. The composition of extracts was studied using HPLC-MS. Both MTT and FC found the cytotoxic effect in 6 of10 tested cyanobacterial extracts, but these results did not correlate with those obtained by brine shrimp assay. In Anabaena torulosa,Cylindrospermum sp. the most severe damage was recorded by both methods. In cells treated by the extract of A. torulosa, 100% inhibition was found already after 60-minute exposure. Induction of necrosis was revealed by FC, since all tested cells were marked by propidium iodide indicating disruption of the cellular membrane.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EH - Ekologie – společenstva

OECD FORD obor

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Projekt

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Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2005

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Czech Phycology

ISSN

1213-3434

e-ISSN

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Svazek periodika

5

Číslo periodika v rámci svazku

-

Stát vydavatele periodika

CZ - Česká republika

Počet stran výsledku

12

Strana od-do

79-90

Kód UT WoS článku

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EID výsledku v databázi Scopus

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