Karyotyp, pohlavní chromatin a diferenciace pohlavních chromosomů u zavíječe datlového, Ectomyelois ceratoniae (Lepidoptera : Pyralidae)

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60077395%3A_____%2F04%3A00104412" target="_blank" >RIV/60077395:_____/04:00104412 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/60076658:12310/04:00008643

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Karyotype, sex chromatin and sex chromosome differentiation in the carob moth, Ectomyelois ceratoniae (Lepidoptera : Pyralidae)

Popis výsledku v původním jazyce

This paper reports results of the first cytogenetic investigation carried out in the carob moth, Ectomyelois ceratoniae Zeller, the serious polyphagous pest of both stored products and field crops in the Mediterranean basin and Near East regions. The carob moth karyotype consists of 2n=62 chromosomes. In pachytene complements, two NOR bivalents were observed. However, we failed to identify sex chromosomes. In order to differentiate the W-Z sex chromosomes, we employed genomic in situ hybridization (GISH) and comparative genomic hybridization (CGH). GISH detected the W chromosome by strong binding of the Cy3-labelled, female-derived DNA probe. With CGH, both the Cy3-labelled female-derived probe and Fluor-X labelled male-derived probe evenly bound to the W. This suggested that the W is composed predominantly of repetitive DNA sequences occurring scattered in other chromosomes but accumulated in the W chromosome

Název v anglickém jazyce

Karyotype, sex chromatin and sex chromosome differentiation in the carob moth, Ectomyelois ceratoniae (Lepidoptera : Pyralidae)

Popis výsledku anglicky

This paper reports results of the first cytogenetic investigation carried out in the carob moth, Ectomyelois ceratoniae Zeller, the serious polyphagous pest of both stored products and field crops in the Mediterranean basin and Near East regions. The carob moth karyotype consists of 2n=62 chromosomes. In pachytene complements, two NOR bivalents were observed. However, we failed to identify sex chromosomes. In order to differentiate the W-Z sex chromosomes, we employed genomic in situ hybridization (GISH) and comparative genomic hybridization (CGH). GISH detected the W chromosome by strong binding of the Cy3-labelled, female-derived DNA probe. With CGH, both the Cy3-labelled female-derived probe and Fluor-X labelled male-derived probe evenly bound to the W. This suggested that the W is composed predominantly of repetitive DNA sequences occurring scattered in other chromosomes but accumulated in the W chromosome

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EB - Genetika a molekulární biologie

OECD FORD obor

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Projekt

<a href="/cs/project/IAA6007307" target="_blank" >IAA6007307: Role pohlavního chromosomu W v determinaci pohlaví motýlů</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2004

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Caryologia

ISSN

0008-7114

e-ISSN

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Svazek periodika

57

Číslo periodika v rámci svazku

2

Stát vydavatele periodika

IT - Italská republika

Počet stran výsledku

10

Strana od-do

184-194

Kód UT WoS článku

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EID výsledku v databázi Scopus

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