Cytogenetika obaleče jablečného: karyotyp, chromosomální lokalizace rDNA a molekulární diferenciace pohlavních chromosomů

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60077395%3A_____%2F05%3A00028510" target="_blank" >RIV/60077395:_____/05:00028510 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/60076658:12310/05:00008704

Výsledek na webu

—

DOI - Digital Object Identifier

—

Jazyk výsledku

angličtina

Název v původním jazyce

Codling moth cytogenetics: karyotype, chromosomal location of rDNA, and molecular differentiation of sex chromosomes

Popis výsledku v původním jazyce

We performed a detailed karyotype analysis in the codling moth, Cydia pomonella, the key pest of pome fruit in the temperate regions of the world. The karyotype consisted of 2n = 56 chromosomes. In pachytene nuclei, a curious NOR (nucleolar organizer region) bivalent was observed. It carried 2 nucleoli, each associated with one end of the bivalent as confirmed by FISH with an 18S ribosomal DNA, which detected 2 clusters of rRNA genes. Pachytene oocytes showed a sex chromosome bivalent (WZ). Molecular differentiation of the sex chromosomes was studied using genomic in situ hybridization (GISH) and comparative genomic hybridization (CGH). GISH detected the W chromosome by strong binding of the female-derived DNA probe. With CGH, both the female-derived probe and male-derived probe evenly bound to the W chromosome. This suggested that the W chromosome is predominantly composed of repetitive DNA sequences occurring scattered in other chromosomes but accumulated in the W chromosome.

Název v anglickém jazyce

Codling moth cytogenetics: karyotype, chromosomal location of rDNA, and molecular differentiation of sex chromosomes

Popis výsledku anglicky

We performed a detailed karyotype analysis in the codling moth, Cydia pomonella, the key pest of pome fruit in the temperate regions of the world. The karyotype consisted of 2n = 56 chromosomes. In pachytene nuclei, a curious NOR (nucleolar organizer region) bivalent was observed. It carried 2 nucleoli, each associated with one end of the bivalent as confirmed by FISH with an 18S ribosomal DNA, which detected 2 clusters of rRNA genes. Pachytene oocytes showed a sex chromosome bivalent (WZ). Molecular differentiation of the sex chromosomes was studied using genomic in situ hybridization (GISH) and comparative genomic hybridization (CGH). GISH detected the W chromosome by strong binding of the female-derived DNA probe. With CGH, both the female-derived probe and male-derived probe evenly bound to the W chromosome. This suggested that the W chromosome is predominantly composed of repetitive DNA sequences occurring scattered in other chromosomes but accumulated in the W chromosome.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EB - Genetika a molekulární biologie

OECD FORD obor

—

Projekt

<a href="/cs/project/IAA6007307" target="_blank" >IAA6007307: Role pohlavního chromosomu W v determinaci pohlaví motýlů</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2005

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Genome

ISSN

0831-2796

e-ISSN

—

Svazek periodika

48

Číslo periodika v rámci svazku

-

Stát vydavatele periodika

CA - Kanada

Počet stran výsledku

10

Strana od-do

1083-1092

Kód UT WoS článku

—

EID výsledku v databázi Scopus

—