Fylogenetická analýza trypanosom sladkovodních ryb Evropy využívající sekvence ssu rRNA genu a polymorfismus náhodně amplifikované DNA

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60077409%3A_____%2F05%3A00025690" target="_blank" >RIV/60077409:_____/05:00025690 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Phylogenetic analysis of freshwater fish trypanosomes from Europe using ssu rRNA gene sequences and random amplification of polymorphic DNA

Popis výsledku v původním jazyce

A collection of 22 cloned trypanosome isolates from 14 species of European freshwater fish and 1 species of African freshwater fish were examined by phylogenetic analysis. SSU rRNA genes of 8 isolates were sequenced and compared with sequences from a wide selection of vertebrate trypanosome isolates. All freshwater isolates fell in a single clade, subdivided into 3 groups. This clade sits within a robust clade of trypanosomes from marine fish, amphibia, reptiles and the duck-billed platypus. All 22 trypanosome clones were analysed by RAPD. The resulting tree shows 3 groups congruent with the groups identified in the ssu rRNA gene phylogeny. Two of these groups contain the majority of isolates. These groups do not separate trypanosome species distinguished by morphology or host origin, and thus these criteria do not appear to be reliable guides to genetic relationships among fish trypanosomes. The two groups themselves may represent different species of fish trypanosomes.

Název v anglickém jazyce

Phylogenetic analysis of freshwater fish trypanosomes from Europe using ssu rRNA gene sequences and random amplification of polymorphic DNA

Popis výsledku anglicky

A collection of 22 cloned trypanosome isolates from 14 species of European freshwater fish and 1 species of African freshwater fish were examined by phylogenetic analysis. SSU rRNA genes of 8 isolates were sequenced and compared with sequences from a wide selection of vertebrate trypanosome isolates. All freshwater isolates fell in a single clade, subdivided into 3 groups. This clade sits within a robust clade of trypanosomes from marine fish, amphibia, reptiles and the duck-billed platypus. All 22 trypanosome clones were analysed by RAPD. The resulting tree shows 3 groups congruent with the groups identified in the ssu rRNA gene phylogeny. Two of these groups contain the majority of isolates. These groups do not separate trypanosome species distinguished by morphology or host origin, and thus these criteria do not appear to be reliable guides to genetic relationships among fish trypanosomes. The two groups themselves may represent different species of fish trypanosomes.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EA - Morfologické obory a cytologie

OECD FORD obor

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Projekt

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Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2005

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Parasitology

ISSN

0031-1820

e-ISSN

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Svazek periodika

130

Číslo periodika v rámci svazku

4

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

8

Strana od-do

405-412

Kód UT WoS článku

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EID výsledku v databázi Scopus

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