Phylogeny and morphological variability of trypanosomes from African pelomedisid turtles with redescription of Trypanosoma mocambicum Pienaar, 1962

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62157124%3A16270%2F15%3A43873852" target="_blank" >RIV/62157124:16270/15:43873852 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/62157124:16170/15:43873852 RIV/62157124:16810/15:43873852

Výsledek na webu

<a href="http://dx.doi.org/10.1016/j.protis.2015.10.002" target="_blank" >http://dx.doi.org/10.1016/j.protis.2015.10.002</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.protis.2015.10.002" target="_blank" >10.1016/j.protis.2015.10.002</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Phylogeny and morphological variability of trypanosomes from African pelomedisid turtles with redescription of Trypanosoma mocambicum Pienaar, 1962

Popis výsledku v původním jazyce

Little is known about host specifity, genetic diversity and phylogenetic relationships of African turtle trypanosomes. Using PCR targeting the SSU rRNA gene, we detected trypanosomes in 24 of 134 (17.9%) wild caught African pelomedusid turtles: Pelusiosupembae (n = 14), P. bechuanicus (n= 1), P. rhodesianus (n = 3) and P. subniger (n = 6). Mixed infection of Trypanosoma species was confirmed by PCR in three specimens of P. upembae, and in one specimen each of P. bechuanicus, P. rhodesianus, and P. subniger. Microscopic examination of stained blood smears revealed two distinct forms (broad and slender) of trypomastigotes. The broad form coincided in morphology with T. mocambicum Pienaar, 1962. Accordingly, we have designated this form as the neotype ofT. mocambicum. In phylogenetic analysis of the SSU rRNA gene, all the new turtle trypanosome sequences grouped in a single clade within the strongly supported "aquatic" clade of Trypanosoma species. The turtle trypanosome clade was furth

Název v anglickém jazyce

Phylogeny and morphological variability of trypanosomes from African pelomedisid turtles with redescription of Trypanosoma mocambicum Pienaar, 1962

Popis výsledku anglicky

Little is known about host specifity, genetic diversity and phylogenetic relationships of African turtle trypanosomes. Using PCR targeting the SSU rRNA gene, we detected trypanosomes in 24 of 134 (17.9%) wild caught African pelomedusid turtles: Pelusiosupembae (n = 14), P. bechuanicus (n= 1), P. rhodesianus (n = 3) and P. subniger (n = 6). Mixed infection of Trypanosoma species was confirmed by PCR in three specimens of P. upembae, and in one specimen each of P. bechuanicus, P. rhodesianus, and P. subniger. Microscopic examination of stained blood smears revealed two distinct forms (broad and slender) of trypomastigotes. The broad form coincided in morphology with T. mocambicum Pienaar, 1962. Accordingly, we have designated this form as the neotype ofT. mocambicum. In phylogenetic analysis of the SSU rRNA gene, all the new turtle trypanosome sequences grouped in a single clade within the strongly supported "aquatic" clade of Trypanosoma species. The turtle trypanosome clade was furth

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EG - Zoologie

OECD FORD obor

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Projekt

<a href="/cs/project/ED1.1.00%2F02.0068" target="_blank" >ED1.1.00/02.0068: CEITEC - central european institute of technology</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2015

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Protist

ISSN

1434-4610

e-ISSN

—

Svazek periodika

166

Číslo periodika v rámci svazku

6

Stát vydavatele periodika

DE - Spolková republika Německo

Počet stran výsledku

10

Strana od-do

599-608

Kód UT WoS článku

000366806600001

EID výsledku v databázi Scopus

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