Studium asymetrické somatické hybridizace u rodu Solanum.

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60109807%3A_____%2F07%3A8P001082" target="_blank" >RIV/60109807:_____/07:8P001082 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

A study of asymetric somatic hybridization in genus Solanum.

Popis výsledku v původním jazyce

Optimalization of protoplast organelles inactivation (nuclei, chloroplasts, mitochondria) with goals using them in cybridization is aim of this study. The effort is focused especially on use extranuclei inheritance of wild species to increase variabilityof cultivated potato. Inactivation of nuclei and semiautonomic organelles was studied in mesophyll protoplasts selected from the members of the genus Solanum tuberosum (cv. Kordoba, cv. Karin, cv. Komtesa) and Solanum verrucosum. Immediately after the isolation, an aliquot of protoplasts was UV-irradiated, these protoplasts did not regenerate. Inactivation of semiautonomic organelles was performed in aliquots of protoplasts with graded concentrations of IODOACETAMIDE (0.3 mM; 0.4 mM), RHODAMINE 6G (0.05 mM; 0.1 mM) and IODOACETIC ACID (0.125 mM; 0.2 mM). These concentrations of active substances did not damage protoplasts and the regeneration of a cell wall was sporadically recorded. In controls, division was observed and microcolonies

Název v anglickém jazyce

A study of asymetric somatic hybridization in genus Solanum.

Popis výsledku anglicky

Optimalization of protoplast organelles inactivation (nuclei, chloroplasts, mitochondria) with goals using them in cybridization is aim of this study. The effort is focused especially on use extranuclei inheritance of wild species to increase variabilityof cultivated potato. Inactivation of nuclei and semiautonomic organelles was studied in mesophyll protoplasts selected from the members of the genus Solanum tuberosum (cv. Kordoba, cv. Karin, cv. Komtesa) and Solanum verrucosum. Immediately after the isolation, an aliquot of protoplasts was UV-irradiated, these protoplasts did not regenerate. Inactivation of semiautonomic organelles was performed in aliquots of protoplasts with graded concentrations of IODOACETAMIDE (0.3 mM; 0.4 mM), RHODAMINE 6G (0.05 mM; 0.1 mM) and IODOACETIC ACID (0.125 mM; 0.2 mM). These concentrations of active substances did not damage protoplasts and the regeneration of a cell wall was sporadically recorded. In controls, division was observed and microcolonies

Druh

O - Ostatní výsledky

CEP obor

EI - Biotechnologie a bionika

OECD FORD obor

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Projekt

<a href="/cs/project/QF4108" target="_blank" >QF4108: Využití techniky fúze protoplastů ve šlechtění hospodářsky významných plodin rodu Brassica, Cucumis a Solanum</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2007

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů