Úvod do cybridizace mezi Solanum verrucosum a Solanum tuberosum

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60109807%3A_____%2F08%3A%230000093" target="_blank" >RIV/60109807:_____/08:#0000093 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Introduction to cybridization between Solanum verrucosum amd Solanum tuberosum

Popis výsledku v původním jazyce

The protoplasts of S. verrucosum PI 161173 were UV-irradiated to inactivate the nuclei after determining the optimal UV dose (germicidal lamp - 254 nm, 370 ?W . cm-2 for 10 min). The cellular organelles of the S. tuberosum (cv. Karin, cv. Komtesa, cv. Kordoba) protoplasts were inactivated. Concentrations (all in mM) of iodoacetamide (IOA; 0.125; 0.25; 0.3; 0.4; 0.5; 1; 2; 5), iodoacetic acid (IOAA; 0.125; 0.2; 0.25) and rhodamine 6G (RH6G; 0.05; 0.1; 0.125; 0.25) were tested and optimal concentrations (0.4 mM IOA and 0.2 mM IOAA) were determined for use in asymmetric somatic hybridization. The UV irradiated protoplasts and the protoplasts with inactivated metabolism were then electrofused. Regeneration of the fused protoplasts and nonfused treated protoplasts (UV or 0.2 mM IOAA or 0.4 mM IOA) were evaluated. Fused protoplasts were more viable and prospered better than treated nonfused controls. Plant regeneration in combination ver (UV) + tbr cv. Karin (0.4 mM IOA) was recorded.

Název v anglickém jazyce

Introduction to cybridization between Solanum verrucosum amd Solanum tuberosum

Popis výsledku anglicky

The protoplasts of S. verrucosum PI 161173 were UV-irradiated to inactivate the nuclei after determining the optimal UV dose (germicidal lamp - 254 nm, 370 ?W . cm-2 for 10 min). The cellular organelles of the S. tuberosum (cv. Karin, cv. Komtesa, cv. Kordoba) protoplasts were inactivated. Concentrations (all in mM) of iodoacetamide (IOA; 0.125; 0.25; 0.3; 0.4; 0.5; 1; 2; 5), iodoacetic acid (IOAA; 0.125; 0.2; 0.25) and rhodamine 6G (RH6G; 0.05; 0.1; 0.125; 0.25) were tested and optimal concentrations (0.4 mM IOA and 0.2 mM IOAA) were determined for use in asymmetric somatic hybridization. The UV irradiated protoplasts and the protoplasts with inactivated metabolism were then electrofused. Regeneration of the fused protoplasts and nonfused treated protoplasts (UV or 0.2 mM IOAA or 0.4 mM IOA) were evaluated. Fused protoplasts were more viable and prospered better than treated nonfused controls. Plant regeneration in combination ver (UV) + tbr cv. Karin (0.4 mM IOA) was recorded.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EI - Biotechnologie a bionika

OECD FORD obor

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Projekt

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Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2008

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Vědecké práce Výzkumný ústav bramborářský Havlíčkův Brod

ISSN

1802-940X

e-ISSN

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Svazek periodika

2008

Číslo periodika v rámci svazku

16

Stát vydavatele periodika

CZ - Česká republika

Počet stran výsledku

11

Strana od-do

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Kód UT WoS článku

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EID výsledku v databázi Scopus

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