Expression of Recombinant Potato leafroll virus Structural and Non-structural Proteins for Antibody Production

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60109807%3A_____%2F11%3A2R000271" target="_blank" >RIV/60109807:_____/11:2R000271 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.1111/j.1439-0434.2010.01740.x" target="_blank" >http://dx.doi.org/10.1111/j.1439-0434.2010.01740.x</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1111/j.1439-0434.2010.01740.x" target="_blank" >10.1111/j.1439-0434.2010.01740.x</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Expression of Recombinant Potato leafroll virus Structural and Non-structural Proteins for Antibody Production

Popis výsledku v původním jazyce

Vector pMPM-A4W and vectors pQE-30 and pET- 45b(+) containing the 6x His-tag sequence were used for expression of Potato leafroll virus (PLRV) structural and non-structural proteins in Escherichia coli. Coat protein (CP) and RNA-dependent RNA polymerase(RdRp)?fragments RdRp43-616 and RdRp304-537 were chosen for expression. A high level of CP and RdRp304-537 was obtained only in an expression system using pET-45b(+) vector and E. coli Rosettagami 2(DE3) cells. After purification, the His-tagged PLRV proteins were used for immunization of rabbits. New Zealand rabbits were immunized either with the recombinant PLRV RdRp304-537 or CP protein,and the antisera obtained were assessed by IPTAELISA using extracts from healthy and PLRV-infected potato plants asantigens. The anti-PLRV CP antibodies were weak and less specific than the commercial PRI antibodies. Antibodies to PLRV non-structural proteins are not available commercially. Although they reacted weakly, our antibodies are the first t

Název v anglickém jazyce

Expression of Recombinant Potato leafroll virus Structural and Non-structural Proteins for Antibody Production

Popis výsledku anglicky

Vector pMPM-A4W and vectors pQE-30 and pET- 45b(+) containing the 6x His-tag sequence were used for expression of Potato leafroll virus (PLRV) structural and non-structural proteins in Escherichia coli. Coat protein (CP) and RNA-dependent RNA polymerase(RdRp)?fragments RdRp43-616 and RdRp304-537 were chosen for expression. A high level of CP and RdRp304-537 was obtained only in an expression system using pET-45b(+) vector and E. coli Rosettagami 2(DE3) cells. After purification, the His-tagged PLRV proteins were used for immunization of rabbits. New Zealand rabbits were immunized either with the recombinant PLRV RdRp304-537 or CP protein,and the antisera obtained were assessed by IPTAELISA using extracts from healthy and PLRV-infected potato plants asantigens. The anti-PLRV CP antibodies were weak and less specific than the commercial PRI antibodies. Antibodies to PLRV non-structural proteins are not available commercially. Although they reacted weakly, our antibodies are the first t

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

GF - Choroby, škůdci, plevely a ochrana rostlin

OECD FORD obor

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Projekt

<a href="/cs/project/QH71123" target="_blank" >QH71123: Variabilita viru svinutky bramboru (PLRV), zvýšení spolehlivosti jeho detekce a uplatnění transgenóze v rezistentním šlechtění.</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2011

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of Phytopathology

ISSN

1439-0434

e-ISSN

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Svazek periodika

159

Číslo periodika v rámci svazku

2

Stát vydavatele periodika

DE - Spolková republika Německo

Počet stran výsledku

3

Strana od-do

130-132

Kód UT WoS článku

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EID výsledku v databázi Scopus

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