Introduction to protoplast culture of Allium genus

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60109807%3A_____%2F12%3A%230000224" target="_blank" >RIV/60109807:_____/12:#0000224 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Introduction to protoplast culture of Allium genus

Popis výsledku v původním jazyce

The aim of this study was to establish the protoplast culture of Allium genus and verify the influence of electric field on regeneration ability of isolated protoplasts. Another goal was tested the possibilities of somatic hybridization in this genus. Mesophyll protoplasts were isolated from in vitro cultured plants of Allium flavum, A. fistulosum, A. schoenoprasum and A. ursinum. The protoplasts were isolated and fused using established protocol for Solanum genus: http://www.vubhb.cz/_te.asp?f=institute/06dogr-protocols-mfpep.htm with prolonged time of enzyme action. The induction of regeneration ability was tested using five parameters of electric field. The regeneration ability of affected protoplasts and fused protoplasts (A. flavum + A. ursinum; A. fistulosum + A. ursinum; A. fistulosum + A. schoenoprasum) was compared with non-affected control. All cultivated protoplasts maintained viability. The cell wall regeneration and cell division were observed in different time and intensi

Název v anglickém jazyce

Introduction to protoplast culture of Allium genus

Popis výsledku anglicky

The aim of this study was to establish the protoplast culture of Allium genus and verify the influence of electric field on regeneration ability of isolated protoplasts. Another goal was tested the possibilities of somatic hybridization in this genus. Mesophyll protoplasts were isolated from in vitro cultured plants of Allium flavum, A. fistulosum, A. schoenoprasum and A. ursinum. The protoplasts were isolated and fused using established protocol for Solanum genus: http://www.vubhb.cz/_te.asp?f=institute/06dogr-protocols-mfpep.htm with prolonged time of enzyme action. The induction of regeneration ability was tested using five parameters of electric field. The regeneration ability of affected protoplasts and fused protoplasts (A. flavum + A. ursinum; A. fistulosum + A. ursinum; A. fistulosum + A. schoenoprasum) was compared with non-affected control. All cultivated protoplasts maintained viability. The cell wall regeneration and cell division were observed in different time and intensi

Druh

O - Ostatní výsledky

CEP obor

EI - Biotechnologie a bionika

OECD FORD obor

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Projekt

<a href="/cs/project/LF11012" target="_blank" >LF11012: Biotechnologický systém produkce klonů Allium ursinum pro potravinářský a farmaceutický průmysl</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2012

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů