Preliminariy Experientce with Protoplast Culture of Allium ursinum.

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60109807%3A_____%2F15%3A%230000351" target="_blank" >RIV/60109807:_____/15:#0000351 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Preliminariy Experientce with Protoplast Culture of Allium ursinum.

Popis výsledku v původním jazyce

Somatic hybridization is a technique for alternative breeding to increase genetic variability. The aim of this work was to establish protoplast culture of Allium ursinum. The initial genotypes originated from three localities. The homogeneity of these populations was tested using RAPD markers. These analyses showed some differences among tested genotypes. According to preliminary experiments, Alliumursinumprotoplasts seemed to be recalcitrant for protoplast cultures. The influence of plantgrow regulators, sugar type and electric field (AC, DC and combination) was tested.With some modifications, the protoplasts were isolated and affected by electric field according toGreplova et al. (2008). The application of indirect current seemed to be useful tostimulate cell division.Sugar type in culture medium appeared to be very important; the combination of glucose with sucrose proved to be the best.Putrescine showed to be crucial for cell viability and regeneration.

Název v anglickém jazyce

Preliminariy Experientce with Protoplast Culture of Allium ursinum.

Popis výsledku anglicky

Somatic hybridization is a technique for alternative breeding to increase genetic variability. The aim of this work was to establish protoplast culture of Allium ursinum. The initial genotypes originated from three localities. The homogeneity of these populations was tested using RAPD markers. These analyses showed some differences among tested genotypes. According to preliminary experiments, Alliumursinumprotoplasts seemed to be recalcitrant for protoplast cultures. The influence of plantgrow regulators, sugar type and electric field (AC, DC and combination) was tested.With some modifications, the protoplasts were isolated and affected by electric field according toGreplova et al. (2008). The application of indirect current seemed to be useful tostimulate cell division.Sugar type in culture medium appeared to be very important; the combination of glucose with sucrose proved to be the best.Putrescine showed to be crucial for cell viability and regeneration.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

GE - Šlechtění rostlin

OECD FORD obor

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Projekt

<a href="/cs/project/LF11012" target="_blank" >LF11012: Biotechnologický systém produkce klonů Allium ursinum pro potravinářský a farmaceutický průmysl</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2015

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Acta Horticulturae

ISSN

0567-7572

e-ISSN

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Svazek periodika

1083

Číslo periodika v rámci svazku

1

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

7

Strana od-do

461-467

Kód UT WoS článku

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EID výsledku v databázi Scopus

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