Construction of a Francisella tularensis two-dimensional electrophoresis protein database

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60162694%3AG16__%2F01%3A00000499" target="_blank" >RIV/60162694:G16__/01:00000499 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/61388971:_____/01:53010124 RIV/60162694:G38__/01:00000499

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Construction of a Francisella tularensis two-dimensional electrophoresis protein database

Popis výsledku v původním jazyce

We have started the construction of a two dimensional database of the proteome of Francisella tularensis, a bacterium that is responsible for the highly pathogenic disease tularemia. The genome of this intracellular pathogen is not completely sequenced,yet and, currently, information only about 54 proteins is available from NCBI database. We have analyzed the Francisella tularensis live vaccine strain by two-dimensional gel electrophoresis with immobilized pH 3-10 gradient in the first dimension and 9-16 % gradient or tricine SDS-PAGE in the second dimension. In both cases about 2000 spots were detected. Furthermore, we compared the protein pattern of the non-virulent Francisella tularensis live vaccine strain with protein profiles of two wild type clinical isolates and more than 50 differentially expressed proteins were counted. The separated proteins are going to be identified by peptide mass fingerprinting, however, due to the lack of complete genome sequence data only eight protei

Název v anglickém jazyce

Construction of a Francisella tularensis two-dimensional electrophoresis protein database

Popis výsledku anglicky

We have started the construction of a two dimensional database of the proteome of Francisella tularensis, a bacterium that is responsible for the highly pathogenic disease tularemia. The genome of this intracellular pathogen is not completely sequenced,yet and, currently, information only about 54 proteins is available from NCBI database. We have analyzed the Francisella tularensis live vaccine strain by two-dimensional gel electrophoresis with immobilized pH 3-10 gradient in the first dimension and 9-16 % gradient or tricine SDS-PAGE in the second dimension. In both cases about 2000 spots were detected. Furthermore, we compared the protein pattern of the non-virulent Francisella tularensis live vaccine strain with protein profiles of two wild type clinical isolates and more than 50 differentially expressed proteins were counted. The separated proteins are going to be identified by peptide mass fingerprinting, however, due to the lack of complete genome sequence data only eight protei

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EC - Imunologie

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2001

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Proteomics

ISSN

1615-9853

e-ISSN

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Svazek periodika

1

Číslo periodika v rámci svazku

4

Stát vydavatele periodika

DE - Spolková republika Německo

Počet stran výsledku

8

Strana od-do

508-515

Kód UT WoS článku

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EID výsledku v databázi Scopus

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