K vytvoření Francisella tularensis 2-DE datebáze

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60162694%3AG38__%2F00%3A00000302" target="_blank" >RIV/60162694:G38__/00:00000302 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Toward the construction of Francisella tularensis 2-DE database

Popis výsledku v původním jazyce

Francisella tularensis is an obligate intracellular pathogen whose genome sequencing will be completed shortly. The next step should be the analysis of functional complement of genetic information so called proteome. Proteom is a new rapidly emerging field of study utilizing the combination of two-dimensional gel electrophoresis (2-DE) and mass spectrometry.We applied 2-DE methodology with immobilized pH gradients in the first dimension for separation of complete protein lysates of attenuated Francisella tularensis strain LVS. Two different techniques 9-16 % gradient SDS-PAGE and Tricine SDS-PAGE were used in the second dimension for the resolution of proteins in the range of molecular weights 10 to 200 kDa and 5-70 kDa, respectively. In the first caseabout 2700 proteins were detected on one gel, regarding Tricine SDS-PAGE we were able to stain approximately 1200 different protein molecules. Besides the cultivation of bacteria under normal condition we also examined the protein expres

Název v anglickém jazyce

Toward the construction of Francisella tularensis 2-DE database

Popis výsledku anglicky

Francisella tularensis is an obligate intracellular pathogen whose genome sequencing will be completed shortly. The next step should be the analysis of functional complement of genetic information so called proteome. Proteom is a new rapidly emerging field of study utilizing the combination of two-dimensional gel electrophoresis (2-DE) and mass spectrometry.We applied 2-DE methodology with immobilized pH gradients in the first dimension for separation of complete protein lysates of attenuated Francisella tularensis strain LVS. Two different techniques 9-16 % gradient SDS-PAGE and Tricine SDS-PAGE were used in the second dimension for the resolution of proteins in the range of molecular weights 10 to 200 kDa and 5-70 kDa, respectively. In the first caseabout 2700 proteins were detected on one gel, regarding Tricine SDS-PAGE we were able to stain approximately 1200 different protein molecules. Besides the cultivation of bacteria under normal condition we also examined the protein expres

Druh

D - Stať ve sborníku

CEP obor

EC - Imunologie

OECD FORD obor

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Projekt

<a href="/cs/project/LN00A033" target="_blank" >LN00A033: Proteomové centrum pro studium intracelulárního parazitizmu bakterií</a><br>

Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2000

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

The 3rd International Conference on Tularemia

ISBN

1104-9154

ISSN

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e-ISSN

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Počet stran výsledku

2

Strana od-do

29-30

Název nakladatele

FOA Defence Research Establishment

Místo vydání

Umea

Místo konání akce

Umea, Sweden

Datum konání akce

27. 8. 2000

Typ akce podle státní příslušnosti

WRD - Celosvětová akce

Kód UT WoS článku

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