Induction and characterization of a replication competent cervid endogenous gammaretrovirus (CrERV) from mule deer cells
Identifikátory výsledku
Kód výsledku v IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60162694%3AG33__%2F15%3AN0000002" target="_blank" >RIV/60162694:G33__/15:N0000002 - isvavai.cz</a>
Nalezeny alternativní kódy
RIV/68378050:_____/15:00455200
Výsledek na webu
<a href="http://hdl.handle.net/11104/0255872" target="_blank" >http://hdl.handle.net/11104/0255872</a>
DOI - Digital Object Identifier
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Alternativní jazyky
Jazyk výsledku
angličtina
Název v původním jazyce
Induction and characterization of a replication competent cervid endogenous gammaretrovirus (CrERV) from mule deer cells
Popis výsledku v původním jazyce
Endogenous retroviruses (ERVs) were acquired during evolution of their host organisms after infection and mendelian inheritance in the germline by their exogenous counterparts. The ERVs can spread in the host genome and in some cases they affect the host phenotype. The cervid endogenous gammaretrovirus (CrERV) is one of only a few well-defined examples of evolutionarily recent invasion of mammalian genome by retroviruses. Thousands of insertionally polymorphic CrERV integration sites have been detected in wild ranging mule deer (Odocoileus hemionus) host populations. Here, we describe for the first time induction of replication competent CrERV by cocultivation of deer and human cells. We characterize the physical properties and tropism of the induced virus. The genomic sequence of the induced virus is phylogenetically related to the evolutionarily young endogenous CrERVs described so far. We also describe the level of replication block of CrERV on deer cells and its capacity to establish superinfection interference. (C) 2015 Elsevier Inc. All rights reserved.
Název v anglickém jazyce
Induction and characterization of a replication competent cervid endogenous gammaretrovirus (CrERV) from mule deer cells
Popis výsledku anglicky
Endogenous retroviruses (ERVs) were acquired during evolution of their host organisms after infection and mendelian inheritance in the germline by their exogenous counterparts. The ERVs can spread in the host genome and in some cases they affect the host phenotype. The cervid endogenous gammaretrovirus (CrERV) is one of only a few well-defined examples of evolutionarily recent invasion of mammalian genome by retroviruses. Thousands of insertionally polymorphic CrERV integration sites have been detected in wild ranging mule deer (Odocoileus hemionus) host populations. Here, we describe for the first time induction of replication competent CrERV by cocultivation of deer and human cells. We characterize the physical properties and tropism of the induced virus. The genomic sequence of the induced virus is phylogenetically related to the evolutionarily young endogenous CrERVs described so far. We also describe the level of replication block of CrERV on deer cells and its capacity to establish superinfection interference. (C) 2015 Elsevier Inc. All rights reserved.
Klasifikace
Druh
J<sub>x</sub> - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)
CEP obor
EB - Genetika a molekulární biologie
OECD FORD obor
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Návaznosti výsledku
Projekt
<a href="/cs/project/LK11215" target="_blank" >LK11215: Regulace aktivních endogenních retrovirů v savčím genomu</a><br>
Návaznosti
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Ostatní
Rok uplatnění
2015
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Údaje specifické pro druh výsledku
Název periodika
Virology
ISSN
0042-6822
e-ISSN
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Svazek periodika
485
Číslo periodika v rámci svazku
November
Stát vydavatele periodika
US - Spojené státy americké
Počet stran výsledku
8
Strana od-do
96-103
Kód UT WoS článku
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EID výsledku v databázi Scopus
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