Strukturální proteinová analýza polyvalentního Stafylokokového bakteriofága 812

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60162694%3AG44__%2F06%3A00001477" target="_blank" >RIV/60162694:G44__/06:00001477 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00216224:14310/07:00020066 RIV/60162694:G44__/07:00001855

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Structural protein analysis of the polyvalent staphylococcal bacteriophage 812

Popis výsledku v původním jazyce

Phage 812 is a polyvalent phage with very broad host range in the genus Staphylococcus, which makes it a suitable candidate for phage therapy of staphylococcal infections. This proteomic study, combining the results of both one- and two-dimensional electrophoreses (1-DE, 2-DE) followed by peptide mass fingerprinting (PMF), led to the identification of 24 virion proteins. Twenty new proteins, not yet identified by proteome analysis of closely related staphylococcal phages K and G1, were identified usingthis approach. Fifteen proteins were assigned unambiguously to the head-tail genome module; the remaining nine proteins are encoded by genes of the left or right arms of the phage genome. As expected, the most abundant proteins in the electrophoretic patterns are the major capsid protein, the major tail sheath protein, and proteins identical to ORF 50 and ORF 95 of phage K, although their function is only putative. Identification of these twenty new proteins contributes substantially to

Název v anglickém jazyce

Structural protein analysis of the polyvalent staphylococcal bacteriophage 812

Popis výsledku anglicky

Phage 812 is a polyvalent phage with very broad host range in the genus Staphylococcus, which makes it a suitable candidate for phage therapy of staphylococcal infections. This proteomic study, combining the results of both one- and two-dimensional electrophoreses (1-DE, 2-DE) followed by peptide mass fingerprinting (PMF), led to the identification of 24 virion proteins. Twenty new proteins, not yet identified by proteome analysis of closely related staphylococcal phages K and G1, were identified usingthis approach. Fifteen proteins were assigned unambiguously to the head-tail genome module; the remaining nine proteins are encoded by genes of the left or right arms of the phage genome. As expected, the most abundant proteins in the electrophoretic patterns are the major capsid protein, the major tail sheath protein, and proteins identical to ORF 50 and ORF 95 of phage K, although their function is only putative. Identification of these twenty new proteins contributes substantially to

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EE - Mikrobiologie, virologie

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2007

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Proteomics

ISSN

1615-9853

e-ISSN

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Svazek periodika

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Číslo periodika v rámci svazku

6

Stát vydavatele periodika

DE - Spolková republika Německo

Počet stran výsledku

9

Strana od-do

64-72

Kód UT WoS článku

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EID výsledku v databázi Scopus

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