Proteomická analýza odpovědi Francisella tularensis LVS na nedostatek železa: indukce F. tularensis proteinů ostrova patogenity IglABC

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60162694%3AG44__%2F07%3A00001875" target="_blank" >RIV/60162694:G44__/07:00001875 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Proteomics analysis of the Francisella tularensis LVS response to iron restriction: induction of the F. tularensis pathogenicity island proteins IglABC

Popis výsledku v původním jazyce

Francisella tularensis is a highly virulent, facultative intracellular pathogen that causes tularemia in humans and animals. Although it is one of the most infectious bacterial pathogens, little is known about its virulence mechanisms. In the present study we investigated the response of F. tularensis live vaccine strain (LVS) to iron depletion, which simulates the environment within the host. In order to detect alterations in protein synthesis we used metabolic labeling followed by 2D-PAGE analysis. Globally, we detected 141 protein spots whose levels were significantly altered in the iron restricted medium. About 65% of the spots were successfully identified using mass spectrometric approaches. Importantly, among the proteins produced at increased level during iron-limited growth, we found three proteins encoded by the igl operon, located in the Francisella tularensis pathogenicity island I (FPI). Of these, the IglC and IglA proteins were previously reported to be necessary for full

Název v anglickém jazyce

Proteomics analysis of the Francisella tularensis LVS response to iron restriction: induction of the F. tularensis pathogenicity island proteins IglABC

Popis výsledku anglicky

Francisella tularensis is a highly virulent, facultative intracellular pathogen that causes tularemia in humans and animals. Although it is one of the most infectious bacterial pathogens, little is known about its virulence mechanisms. In the present study we investigated the response of F. tularensis live vaccine strain (LVS) to iron depletion, which simulates the environment within the host. In order to detect alterations in protein synthesis we used metabolic labeling followed by 2D-PAGE analysis. Globally, we detected 141 protein spots whose levels were significantly altered in the iron restricted medium. About 65% of the spots were successfully identified using mass spectrometric approaches. Importantly, among the proteins produced at increased level during iron-limited growth, we found three proteins encoded by the igl operon, located in the Francisella tularensis pathogenicity island I (FPI). Of these, the IglC and IglA proteins were previously reported to be necessary for full

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EE - Mikrobiologie, virologie

OECD FORD obor

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Projekt

<a href="/cs/project/OPUOFVZ200602" target="_blank" >OPUOFVZ200602: BOJAGENS - Faktory virulence intracelulárního patogena Francisella tularensis zařazeného do kategorie A bojových biologických agens</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2007

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

FEMS Microbiology Letters

ISSN

0378-1097

e-ISSN

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Svazek periodika

269

Číslo periodika v rámci svazku

1

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

11

Strana od-do

11-21

Kód UT WoS článku

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EID výsledku v databázi Scopus

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