Sensitivity of porcine peripheral blood leukocytes to gamma-irradiation in vivo, in vitro and ex vivo

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60162694%3AG44__%2F11%3A00002445" target="_blank" >RIV/60162694:G44__/11:00002445 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.3109/09553002.2011.548437" target="_blank" >http://dx.doi.org/10.3109/09553002.2011.548437</a>

DOI - Digital Object Identifier

—

Jazyk výsledku

angličtina

Název v původním jazyce

Sensitivity of porcine peripheral blood leukocytes to gamma-irradiation in vivo, in vitro and ex vivo

Popis výsledku v původním jazyce

The large white pig is a useful experimental model to compare in vivo, in vitro and ex vivo sensitivity of peripheral blood leukocytes to ionizing radiation. We analysed dose- and time-dependent changes of lymphocyte and granulocyte absolute numbers. A flow cytometer were used to determine major leukocyte subpopulation numbers in blood samples after red cell removal. Similar to other species, lymphocyte numbers significantly decreased in pigs both in vivo and in vitro in a dose-dependent manner. Our data clearly show that reduction of lymphocyte numbers after irradiation in vivo proceeds much faster than after irradiation in vitro and that granulocyte changes depend only on the time of analysis after irradiation. All experimental arrangements demonstrated the radiosensitivity of lymphocytes and the radioresistance of peripheral blood granulocytes. These in vivo and in vitro approaches, as well as the newly introduced ex vivo observations, appear to be relevant to biodosimetry.

Název v anglickém jazyce

Sensitivity of porcine peripheral blood leukocytes to gamma-irradiation in vivo, in vitro and ex vivo

Popis výsledku anglicky

The large white pig is a useful experimental model to compare in vivo, in vitro and ex vivo sensitivity of peripheral blood leukocytes to ionizing radiation. We analysed dose- and time-dependent changes of lymphocyte and granulocyte absolute numbers. A flow cytometer were used to determine major leukocyte subpopulation numbers in blood samples after red cell removal. Similar to other species, lymphocyte numbers significantly decreased in pigs both in vivo and in vitro in a dose-dependent manner. Our data clearly show that reduction of lymphocyte numbers after irradiation in vivo proceeds much faster than after irradiation in vitro and that granulocyte changes depend only on the time of analysis after irradiation. All experimental arrangements demonstrated the radiosensitivity of lymphocytes and the radioresistance of peripheral blood granulocytes. These in vivo and in vitro approaches, as well as the newly introduced ex vivo observations, appear to be relevant to biodosimetry.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EC - Imunologie

OECD FORD obor

—

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2011

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

International Journal of Radiation Biology

ISSN

0955-3002

e-ISSN

—

Svazek periodika

87

Číslo periodika v rámci svazku

5

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

8

Strana od-do

491-498

Kód UT WoS článku

000289915200006

EID výsledku v databázi Scopus

—