Dynamics of apoptosis in gamma-irradiated lymphocytes and the CD21(+)CD27(+) B-cell subset as a potential biodosimetric marker

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60162694%3AG44__%2F11%3A00002454" target="_blank" >RIV/60162694:G44__/11:00002454 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.1016/j.radmeas.2011.04.005" target="_blank" >http://dx.doi.org/10.1016/j.radmeas.2011.04.005</a>

DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Dynamics of apoptosis in gamma-irradiated lymphocytes and the CD21(+)CD27(+) B-cell subset as a potential biodosimetric marker

Popis výsledku v původním jazyce

The dynamics of apoptosis progression has been studied in human peripheral blood mononuclear cells (PBMC) irradiated with selected doses (0.5, 1, 2, 4, 6, 8 and 10 Gy) of 60Co gamma rays and subsequently cultivated for 16 h in vitro. A dose-dependent increase and decrease of shrunk and intact cells, respectively, has been observed in the range of 0.5-4 Gy while higher doses have not affected the relative numbers of intact and apoptotic cells. The relative number of early apoptotic cells did not change within the dose range studied. Immunophenotyping of intact and very early apoptotic cells have indicated that the remaining intact cells represent the most convenient population for radioresistance studies in vitro. The CD21(+)CD27(+) subset of peripheralB-cells has thus been suggested as a putative biodosimetric marker in vitro.

Název v anglickém jazyce

Dynamics of apoptosis in gamma-irradiated lymphocytes and the CD21(+)CD27(+) B-cell subset as a potential biodosimetric marker

Popis výsledku anglicky

The dynamics of apoptosis progression has been studied in human peripheral blood mononuclear cells (PBMC) irradiated with selected doses (0.5, 1, 2, 4, 6, 8 and 10 Gy) of 60Co gamma rays and subsequently cultivated for 16 h in vitro. A dose-dependent increase and decrease of shrunk and intact cells, respectively, has been observed in the range of 0.5-4 Gy while higher doses have not affected the relative numbers of intact and apoptotic cells. The relative number of early apoptotic cells did not change within the dose range studied. Immunophenotyping of intact and very early apoptotic cells have indicated that the remaining intact cells represent the most convenient population for radioresistance studies in vitro. The CD21(+)CD27(+) subset of peripheralB-cells has thus been suggested as a putative biodosimetric marker in vitro.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EC - Imunologie

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2011

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Radiation Measurements

ISSN

1350-4487

e-ISSN

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Svazek periodika

46

Číslo periodika v rámci svazku

9

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

5

Strana od-do

1009-1013

Kód UT WoS článku

000296039300055

EID výsledku v databázi Scopus

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