Assessment of acetylcholinesterase activity using Indoxylacetate and comparison with the standard Ellman's method

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60162694%3AG44__%2F11%3A00002493" target="_blank" >RIV/60162694:G44__/11:00002493 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.3390/ijms12042631" target="_blank" >http://dx.doi.org/10.3390/ijms12042631</a>

DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Assessment of acetylcholinesterase activity using Indoxylacetate and comparison with the standard Ellman's method

Popis výsledku v původním jazyce

Assay of acetylcholinesterase (AChE) activity plays an important role in diagnostic, detection of pesticides and nerve agents, in vitro characterization of toxins and drugs including potential treatments for Alzheimer's disease. These experiments were done in order to determine whether indoxylacetate could be an adequate chromogenic reactant for AChE assay evaluation. Moreover, the results were compared to the standard Ellman's method. We calculated Michaelis constant Km (2.06 x 10-4 mol/L for acetylthiocholine and 3.21 x 10-3 mol/L for indoxylacetate) maximum reaction velocity Vmax (4.97 x 10-7 kat for acetylcholine and 7.71 x 10-8 kat for indoxylacetate) for electric eel AChE. In a second part, inhibition values were plotted for paraoxon, and reactivation efficacy was measured for some standard oxime reactivators: obidoxime, pralidoxime (2-PAM) and HI-6. Though indoxylacetate is split with lower turnover rate, this compound appears as a very attractive reactant since it does not show

Název v anglickém jazyce

Assessment of acetylcholinesterase activity using Indoxylacetate and comparison with the standard Ellman's method

Popis výsledku anglicky

Assay of acetylcholinesterase (AChE) activity plays an important role in diagnostic, detection of pesticides and nerve agents, in vitro characterization of toxins and drugs including potential treatments for Alzheimer's disease. These experiments were done in order to determine whether indoxylacetate could be an adequate chromogenic reactant for AChE assay evaluation. Moreover, the results were compared to the standard Ellman's method. We calculated Michaelis constant Km (2.06 x 10-4 mol/L for acetylthiocholine and 3.21 x 10-3 mol/L for indoxylacetate) maximum reaction velocity Vmax (4.97 x 10-7 kat for acetylcholine and 7.71 x 10-8 kat for indoxylacetate) for electric eel AChE. In a second part, inhibition values were plotted for paraoxon, and reactivation efficacy was measured for some standard oxime reactivators: obidoxime, pralidoxime (2-PAM) and HI-6. Though indoxylacetate is split with lower turnover rate, this compound appears as a very attractive reactant since it does not show

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

CE - Biochemie

OECD FORD obor

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Projekt

<a href="/cs/project/OVUOFVZ200807" target="_blank" >OVUOFVZ200807: PROTEIN - Biosenzory pro stanovení nervově paralytických a zpuchýřujících látek za použití rekombinantních proteinů a nanotechnologií</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2011

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

International Journal of Molecular Sciences

ISSN

1422-0067

e-ISSN

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Svazek periodika

12

Číslo periodika v rámci svazku

4

Stát vydavatele periodika

CH - Švýcarská konfederace

Počet stran výsledku

10

Strana od-do

2631-2640

Kód UT WoS článku

000289974600011

EID výsledku v databázi Scopus

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