Acetylcholinesterase based dipsticks with indoxylacetate as a substrate for assay of organophosphates and carbamates

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60162694%3AG44__%2F12%3A43874622" target="_blank" >RIV/60162694:G44__/12:43874622 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.1080/00032719.2011.644743" target="_blank" >http://dx.doi.org/10.1080/00032719.2011.644743</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1080/00032719.2011.644743" target="_blank" >10.1080/00032719.2011.644743</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Acetylcholinesterase based dipsticks with indoxylacetate as a substrate for assay of organophosphates and carbamates

Popis výsledku v původním jazyce

Enzyme acetylcholinesterase (AChE) is an important part of cholinergic neurotransmission. It is targeted by many toxins such as nerve agents, organophosphates, and carbamate pesticides. Several drugs for treatment of Alzheimer's disease and Myasthenia gravis are also AChE inhibitors. The inhibitory effect can be used for assay purposes. The presented experiment is devoted to the construction of a colorimetric dipstick with immobilized AChE and using indoxylacetate as a chromogenic substrate. Standard qualitative cellulose filter papers, high-performance TLC plates, cotton gauze, and parafilm were chosen as matrices for the testing. The constructed dipsticks were created for assays of paraoxon as a model organophosphate pesticide and neostigmine as a model carbamate. The assessed limit of detection was 10(-7) mol/l for both inhibitors. It responds absolutely by detecting 4 pmol of inhibitor when a sample volume of 40 mu L is considered. Long term stability and optimization of immobiliza

Název v anglickém jazyce

Acetylcholinesterase based dipsticks with indoxylacetate as a substrate for assay of organophosphates and carbamates

Popis výsledku anglicky

Enzyme acetylcholinesterase (AChE) is an important part of cholinergic neurotransmission. It is targeted by many toxins such as nerve agents, organophosphates, and carbamate pesticides. Several drugs for treatment of Alzheimer's disease and Myasthenia gravis are also AChE inhibitors. The inhibitory effect can be used for assay purposes. The presented experiment is devoted to the construction of a colorimetric dipstick with immobilized AChE and using indoxylacetate as a chromogenic substrate. Standard qualitative cellulose filter papers, high-performance TLC plates, cotton gauze, and parafilm were chosen as matrices for the testing. The constructed dipsticks were created for assays of paraoxon as a model organophosphate pesticide and neostigmine as a model carbamate. The assessed limit of detection was 10(-7) mol/l for both inhibitors. It responds absolutely by detecting 4 pmol of inhibitor when a sample volume of 40 mu L is considered. Long term stability and optimization of immobiliza

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

CB - Analytická chemie, separace

OECD FORD obor

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Projekt

—

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2012

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Analytical Letters

ISSN

0003-2719

e-ISSN

—

Svazek periodika

45

Číslo periodika v rámci svazku

4

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

8

Strana od-do

367-374

Kód UT WoS článku

000301869400006

EID výsledku v databázi Scopus

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