Colorimetric dipstick for assay of organophosphate pesticides and nerve agents represented by paraoxon, sarin and VX

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62157124%3A16270%2F10%3A00002841" target="_blank" >RIV/62157124:16270/10:00002841 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/60162694:G44__/10:00002304 RIV/00216208:11160/10:00300631 RIV/25656180:_____/10:#0000009

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Colorimetric dipstick for assay of organophosphate pesticides and nerve agents represented by paraoxon, sarin and VX

Popis výsledku v původním jazyce

A dipstick for fast assay of nerve agents and organophosphate pesticides was developed. Indicator pH papers were used as detectors. The principle of the assay is based on enzymatic hydrolysis of acetylcholine into acetic acid and choline by acetylcholinesterase. Acidification of the reaction medium due to accumulation of acetic acid was visible. The colour changed from dark red to yellow as the pH indicator recognized pH shift. Presence of an organophosphate pesticide or a nerve agent results in irreversible inhibition of acetylcholinesterase intercepted on the dipstick. The inhibition stops the enzymatic reaction. The inhibition appears as no change of the medium pH. Three compounds were assayed: paraoxon-ethyl as representative organophosphate pesticides and nerve agents sarin and VX. The achieved limit of detection was 5 x 10(-8) M for paraoxon-ethyl and 5 x 10(-9) M for sarin and VX. Dipsticks were found stable for at least one month. Suitability of these dipsticks for routine assa

Název v anglickém jazyce

Colorimetric dipstick for assay of organophosphate pesticides and nerve agents represented by paraoxon, sarin and VX

Popis výsledku anglicky

A dipstick for fast assay of nerve agents and organophosphate pesticides was developed. Indicator pH papers were used as detectors. The principle of the assay is based on enzymatic hydrolysis of acetylcholine into acetic acid and choline by acetylcholinesterase. Acidification of the reaction medium due to accumulation of acetic acid was visible. The colour changed from dark red to yellow as the pH indicator recognized pH shift. Presence of an organophosphate pesticide or a nerve agent results in irreversible inhibition of acetylcholinesterase intercepted on the dipstick. The inhibition stops the enzymatic reaction. The inhibition appears as no change of the medium pH. Three compounds were assayed: paraoxon-ethyl as representative organophosphate pesticides and nerve agents sarin and VX. The achieved limit of detection was 5 x 10(-8) M for paraoxon-ethyl and 5 x 10(-9) M for sarin and VX. Dipsticks were found stable for at least one month. Suitability of these dipsticks for routine assa

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

CB - Analytická chemie, separace

OECD FORD obor

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Projekt

<a href="/cs/project/FR-TI1%2F541" target="_blank" >FR-TI1/541: *Příprava průkazníku pro stanovení organofosfátů</a><br>

Návaznosti

V - Vyzkumna aktivita podporovana z jinych verejnych zdroju

Rok uplatnění

2010

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Talanta

ISSN

0039-9140

e-ISSN

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Svazek periodika

81

Číslo periodika v rámci svazku

1-2

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

4

Strana od-do

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Kód UT WoS článku

000276052700095

EID výsledku v databázi Scopus

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