Electrochemical biosensor based on acetylcholinesterase and indoxylacetate for assay of neurotoxic compounds represented by paraoxon

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43210%2F12%3A00182628" target="_blank" >RIV/62156489:43210/12:00182628 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00216305:26620/12:PU97494 RIV/60162694:G44__/12:43874615

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Electrochemical biosensor based on acetylcholinesterase and indoxylacetate for assay of neurotoxic compounds represented by paraoxon

Popis výsledku v původním jazyce

Acetylcholinesterase (AChE) is an enzyme terminating neurotransmission in cholinergic nervous system. It is targeted by several drugs and toxins. From a toxicological point of view, pesticides and nerve agents are probably the most toxic compounds inhibiting AChE activity. On the account of the enzyme inhibition, AChE is suitable for an in vitro assay of inhibitors and analytical devices such as biosensors are known to work on principle of recording of AChE residual activity. Here reported experiment isbased on previously performed colorimetric dipsticks. Presently, it is further improved by an electrochemical assay. The dipsticks are prepared by an immobilization of electric eel AChE into gelatin membrane on cellulose matrix and assay of the enzyme activity by sticking zone with AChE and indoxylacetate as a chromogenic substrate. The zone with immobilized AChE was placed onto screen printed electrode and enzyme reaction was assessed electrochemically using square wave voltammetry. In

Název v anglickém jazyce

Electrochemical biosensor based on acetylcholinesterase and indoxylacetate for assay of neurotoxic compounds represented by paraoxon

Popis výsledku anglicky

Acetylcholinesterase (AChE) is an enzyme terminating neurotransmission in cholinergic nervous system. It is targeted by several drugs and toxins. From a toxicological point of view, pesticides and nerve agents are probably the most toxic compounds inhibiting AChE activity. On the account of the enzyme inhibition, AChE is suitable for an in vitro assay of inhibitors and analytical devices such as biosensors are known to work on principle of recording of AChE residual activity. Here reported experiment isbased on previously performed colorimetric dipsticks. Presently, it is further improved by an electrochemical assay. The dipsticks are prepared by an immobilization of electric eel AChE into gelatin membrane on cellulose matrix and assay of the enzyme activity by sticking zone with AChE and indoxylacetate as a chromogenic substrate. The zone with immobilized AChE was placed onto screen printed electrode and enzyme reaction was assessed electrochemically using square wave voltammetry. In

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

CB - Analytická chemie, separace

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2012

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

International Journal of Electrochemical Science

ISSN

1452-3981

e-ISSN

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Svazek periodika

7

Číslo periodika v rámci svazku

1

Stát vydavatele periodika

RS - Srbská republika

Počet stran výsledku

8

Strana od-do

50-57

Kód UT WoS článku

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EID výsledku v databázi Scopus

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