Biosensors containing acetylcholinesterase and butyrylcholinesterase as recognition tools for detection of various compounds
Identifikátory výsledku
Kód výsledku v IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60162694%3AG44__%2F15%3A43875342" target="_blank" >RIV/60162694:G44__/15:43875342 - isvavai.cz</a>
Výsledek na webu
<a href="http://www.degruyter.com/view/j/chempap.2015.69.issue-1/s11696-014-0542-x/s11696-014-0542-x.xml" target="_blank" >http://www.degruyter.com/view/j/chempap.2015.69.issue-1/s11696-014-0542-x/s11696-014-0542-x.xml</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.2478/s11696-014-0542-x" target="_blank" >10.2478/s11696-014-0542-x</a>
Alternativní jazyky
Jazyk výsledku
angličtina
Název v původním jazyce
Biosensors containing acetylcholinesterase and butyrylcholinesterase as recognition tools for detection of various compounds
Popis výsledku v původním jazyce
Acetylcholinesterase (AChE) and butyrylcholinesterase (BChE) are enzymes expressed in the human body under physiological conditions. AChE is an important part of the cholinergic nerves where it hydrolyses neurotransmitter acetylcholine. Both cholinesterases are sensitive to inhibitors acting as neurotoxic compounds. In analytical applications, the enzymes can serve as a biorecognition element in biosensors as well as simple disposable sensors (dipsticks) and be used for assaying the neurotoxic compounds. In the present review, the mechanism of AChE and BChE inhibition by disparate compounds is explained and methods for assaying the enzymes activity are shown. Optical, electrochemical, and piezoelectric biosensors are described. Attention is also givento the application of sol-gel techniques and quantum dots in the biosensors' construction. Examples of the biosensors are provided and the pros and cons are discussed. (C) 2014 Institute of Chemistry, Slovak Academy of Sciences
Název v anglickém jazyce
Biosensors containing acetylcholinesterase and butyrylcholinesterase as recognition tools for detection of various compounds
Popis výsledku anglicky
Acetylcholinesterase (AChE) and butyrylcholinesterase (BChE) are enzymes expressed in the human body under physiological conditions. AChE is an important part of the cholinergic nerves where it hydrolyses neurotransmitter acetylcholine. Both cholinesterases are sensitive to inhibitors acting as neurotoxic compounds. In analytical applications, the enzymes can serve as a biorecognition element in biosensors as well as simple disposable sensors (dipsticks) and be used for assaying the neurotoxic compounds. In the present review, the mechanism of AChE and BChE inhibition by disparate compounds is explained and methods for assaying the enzymes activity are shown. Optical, electrochemical, and piezoelectric biosensors are described. Attention is also givento the application of sol-gel techniques and quantum dots in the biosensors' construction. Examples of the biosensors are provided and the pros and cons are discussed. (C) 2014 Institute of Chemistry, Slovak Academy of Sciences
Klasifikace
Druh
J<sub>x</sub> - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)
CEP obor
CB - Analytická chemie, separace
OECD FORD obor
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Návaznosti výsledku
Projekt
<a href="/cs/project/LH11023" target="_blank" >LH11023: Vylepšení vakcinační účinnosti pomocí cholinergní protizánětlivé dráhy</a><br>
Návaznosti
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Ostatní
Rok uplatnění
2015
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Údaje specifické pro druh výsledku
Název periodika
Chemical Papers-Chemicke Zvesti
ISSN
0366-6352
e-ISSN
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Svazek periodika
69
Číslo periodika v rámci svazku
1
Stát vydavatele periodika
SK - Slovenská republika
Počet stran výsledku
13
Strana od-do
4-16
Kód UT WoS článku
000350130100002
EID výsledku v databázi Scopus
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