Carbofuran assay using gelatin based biosensor with acetylcholinesterase as a recognition element

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43210%2F13%3A00199025" target="_blank" >RIV/62156489:43210/13:00199025 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/60162694:G44__/13:43874959 RIV/00216305:26620/13:PU103103 RIV/25766872:_____/12:#0000083

Výsledek na webu

—

DOI - Digital Object Identifier

—

Jazyk výsledku

angličtina

Název v původním jazyce

Carbofuran assay using gelatin based biosensor with acetylcholinesterase as a recognition element

Popis výsledku v původním jazyce

Carbofuran is a neurotoxic pesticide acting as an inhibitor of enzyme acetylcholinesterase (AChE). The fact that carbofuran is able to inhibit AChE can be used for its assay using an appropriate sensor. Here, electrochemical biosensor having immobilizedAChE and using acetylthiocholine as substrate was performed. The biosensor was consisted from a layer containing electric eel AChE and screen printed sensors. AChE was immobilized into gelatin membrane. The immobilization procedure was optimized considering temperature of drying and concentration of gelatin in mixture. Long term stability and interference of organic solvent was done as well. In the final experiment, carbofuran was assayed using the prepared biosensor. Limit of detection 5.01 x 10-10 mol/l (0.111 ng/ml) was achieved.

Název v anglickém jazyce

Carbofuran assay using gelatin based biosensor with acetylcholinesterase as a recognition element

Popis výsledku anglicky

Carbofuran is a neurotoxic pesticide acting as an inhibitor of enzyme acetylcholinesterase (AChE). The fact that carbofuran is able to inhibit AChE can be used for its assay using an appropriate sensor. Here, electrochemical biosensor having immobilizedAChE and using acetylthiocholine as substrate was performed. The biosensor was consisted from a layer containing electric eel AChE and screen printed sensors. AChE was immobilized into gelatin membrane. The immobilization procedure was optimized considering temperature of drying and concentration of gelatin in mixture. Long term stability and interference of organic solvent was done as well. In the final experiment, carbofuran was assayed using the prepared biosensor. Limit of detection 5.01 x 10-10 mol/l (0.111 ng/ml) was achieved.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

CG - Elektrochemie

OECD FORD obor

—

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

O - Projekt operacniho programu

Rok uplatnění

2013

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

International Journal of Electrochemical Science

ISSN

1452-3981

e-ISSN

—

Svazek periodika

8

Číslo periodika v rámci svazku

1

Stát vydavatele periodika

RS - Srbská republika

Počet stran výsledku

9

Strana od-do

71-79

Kód UT WoS článku

—

EID výsledku v databázi Scopus

—