Deletion of IglH in virulent Francisella tularensis subsp. tularensis holarctica FSC200 strain results in attenuation and provides protection against the challenge with the parental strain

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60162694%3AG44__%2F12%3A00002441" target="_blank" >RIV/60162694:G44__/12:00002441 - isvavai.cz</a>

Výsledek na webu

<a href="http://www.journals.elsevier.com/microbes-and-infection/" target="_blank" >http://www.journals.elsevier.com/microbes-and-infection/</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.micinf.2011.08.017" target="_blank" >10.1016/j.micinf.2011.08.017</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Deletion of IglH in virulent Francisella tularensis subsp. tularensis holarctica FSC200 strain results in attenuation and provides protection against the challenge with the parental strain

Popis výsledku v původním jazyce

Francisella tularensis, the causative agent of tularemia, is a highly infectious intracellular pathogen with no licensed vaccine available today. The recent search for genome sequences involved in F. tularensis virulence mechanisms led to the identification of the 30-kb region defined as a Francisella pathogenicity island (FPI). In our previous iTRAQ study we described the concerted upregulation of some FPI proteins in different F. tularensis strains cultivated under stress conditions. Among them we identified the IglH protein whose role in Francisella virulence has not been characterized yet. In this work we deleted the iglH gene in a European clinical isolate of F. tularensis subsp. holarctica FSC200. We showed that the iglH gene is necessary for intracellular growth and escape of F. tularensis from phagosomes. We also showed that the iglH mutant is avirulent in a mouse model of infection and persists in the organs for about three weeks after infection. Importantly, mice vaccinated b

Název v anglickém jazyce

Deletion of IglH in virulent Francisella tularensis subsp. tularensis holarctica FSC200 strain results in attenuation and provides protection against the challenge with the parental strain

Popis výsledku anglicky

Francisella tularensis, the causative agent of tularemia, is a highly infectious intracellular pathogen with no licensed vaccine available today. The recent search for genome sequences involved in F. tularensis virulence mechanisms led to the identification of the 30-kb region defined as a Francisella pathogenicity island (FPI). In our previous iTRAQ study we described the concerted upregulation of some FPI proteins in different F. tularensis strains cultivated under stress conditions. Among them we identified the IglH protein whose role in Francisella virulence has not been characterized yet. In this work we deleted the iglH gene in a European clinical isolate of F. tularensis subsp. holarctica FSC200. We showed that the iglH gene is necessary for intracellular growth and escape of F. tularensis from phagosomes. We also showed that the iglH mutant is avirulent in a mouse model of infection and persists in the organs for about three weeks after infection. Importantly, mice vaccinated b

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EE - Mikrobiologie, virologie

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2012

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Microbes and Infection

ISSN

1286-4579

e-ISSN

—

Svazek periodika

14

Číslo periodika v rámci svazku

2

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

11

Strana od-do

177-187

Kód UT WoS článku

000300819100009

EID výsledku v databázi Scopus

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