Comparison of an Alzheimer disease drug ability to bind acetylcholinesterase using both electrochemical and spectrophotometric assays

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60162694%3AG44__%2F14%3A43875221" target="_blank" >RIV/60162694:G44__/14:43875221 - isvavai.cz</a>

Výsledek na webu

<a href="http://www.roavs.com/pdf-files/Issue-4-2014/203-207.pdf" target="_blank" >http://www.roavs.com/pdf-files/Issue-4-2014/203-207.pdf</a>

DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Comparison of an Alzheimer disease drug ability to bind acetylcholinesterase using both electrochemical and spectrophotometric assays

Popis výsledku v původním jazyce

Acetylcholinesterase (AChE) is an enzyme playing important role in termination of neurotransmission within cholinergic system. The enzyme is targeted by many compounds including nerve agents used for chemical warfare, natural toxins and drugs for Alzheimer disease. Thousands of new inhibitors are prepared for pharmaceutical purposes over the year and necessity to characterize them is given for this reason. In this work, standard spectrophotometric test is compared to electrochemical test in order to assay AChE activity and measure its inhibition. The both test were performed for characterization of an Alzheimer disease drug galantamine. The spectrophotometric test was based on standard Ellman's reaction. Electrochemical test used enzymatic hydrolysis to acetylthiocholine with consequent electrochemical oxidation. In the experiments, we proved that electrochemical test was able to reach better limit of detection for galantamine than the standard spectrophotometric test. Calculated media

Název v anglickém jazyce

Comparison of an Alzheimer disease drug ability to bind acetylcholinesterase using both electrochemical and spectrophotometric assays

Popis výsledku anglicky

Acetylcholinesterase (AChE) is an enzyme playing important role in termination of neurotransmission within cholinergic system. The enzyme is targeted by many compounds including nerve agents used for chemical warfare, natural toxins and drugs for Alzheimer disease. Thousands of new inhibitors are prepared for pharmaceutical purposes over the year and necessity to characterize them is given for this reason. In this work, standard spectrophotometric test is compared to electrochemical test in order to assay AChE activity and measure its inhibition. The both test were performed for characterization of an Alzheimer disease drug galantamine. The spectrophotometric test was based on standard Ellman's reaction. Electrochemical test used enzymatic hydrolysis to acetylthiocholine with consequent electrochemical oxidation. In the experiments, we proved that electrochemical test was able to reach better limit of detection for galantamine than the standard spectrophotometric test. Calculated media

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

CB - Analytická chemie, separace

OECD FORD obor

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Projekt

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Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2014

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Research Opinions in Animal & Veterinary Sciences

ISSN

2221-1896

e-ISSN

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Svazek periodika

4

Číslo periodika v rámci svazku

4

Stát vydavatele periodika

PK - Pákistánská islámská republika

Počet stran výsledku

5

Strana od-do

203-207

Kód UT WoS článku

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EID výsledku v databázi Scopus

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