Determination of peroxidase-like activity of magnetic particles: basic platforms for peroxidase biosensors

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60162694%3AG44__%2F15%3A43875477" target="_blank" >RIV/60162694:G44__/15:43875477 - isvavai.cz</a>

Výsledek na webu

<a href="http://www.electrochemsci.org/papers/vol10/100907033.pdf" target="_blank" >http://www.electrochemsci.org/papers/vol10/100907033.pdf</a>

DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Determination of peroxidase-like activity of magnetic particles: basic platforms for peroxidase biosensors

Popis výsledku v původním jazyce

Magnetic particles (MPs) are a current mean suitable for separation, biosensors construction, nanomedicine etc. They can be easily covered with a biomolecule and used for analytical purposes. Many enzymological assays have to be combined with use peroxidase. In this point of view, glucose assay by glucose oxidase can be exampled. In the present work, we focused our attention on the determination whether MPs based on iron oxide have pseudo-peroxidase activity allowing use them directly without linking to peroxidase. Hydrogen peroxide (H2O2) as the very common substrate of peroxidase was used for this measurement. Michaelis-Menten kinetics was performed for demonstration of catalytic activity of MPs by measuring various concentrations of H2O2 and square wave voltammetry (SWV), cyclic voltammetry (CV) and spectrophotometry were performed for the determination of pseudo-peroxidase activity. The used MPs had significant pseudo-peroxidase activity and Michaelis-Menten like behavior was observed and kinetics constants were determined. Michaelis-Menten constant (Km) was equal to 156.6 - 199.1 mmol/l (values are slightly different for the used analytical methods). Sensitivity of electrochemical and optical methods was also compared resulting in limits of detection found to be 0.42 mmol/l, 0.36 mmol /l and 13.1 mmol /l for SWV, CV and spectrophotometry respectively. In a conclusion, iron oxide MPs have high pseudo-peroxidase activity resulting in opportunity to use them for the determination of hydrogen peroxide. If an oxidase applied as a biorecognition element, there is no need to co-immobilize peroxidase. Km values were also determined for HRP using spectrophotometry (25.1 mmol/l), SWV (167. mmol/l) and CV (163.2 mmol/l).

Název v anglickém jazyce

Determination of peroxidase-like activity of magnetic particles: basic platforms for peroxidase biosensors

Popis výsledku anglicky

Magnetic particles (MPs) are a current mean suitable for separation, biosensors construction, nanomedicine etc. They can be easily covered with a biomolecule and used for analytical purposes. Many enzymological assays have to be combined with use peroxidase. In this point of view, glucose assay by glucose oxidase can be exampled. In the present work, we focused our attention on the determination whether MPs based on iron oxide have pseudo-peroxidase activity allowing use them directly without linking to peroxidase. Hydrogen peroxide (H2O2) as the very common substrate of peroxidase was used for this measurement. Michaelis-Menten kinetics was performed for demonstration of catalytic activity of MPs by measuring various concentrations of H2O2 and square wave voltammetry (SWV), cyclic voltammetry (CV) and spectrophotometry were performed for the determination of pseudo-peroxidase activity. The used MPs had significant pseudo-peroxidase activity and Michaelis-Menten like behavior was observed and kinetics constants were determined. Michaelis-Menten constant (Km) was equal to 156.6 - 199.1 mmol/l (values are slightly different for the used analytical methods). Sensitivity of electrochemical and optical methods was also compared resulting in limits of detection found to be 0.42 mmol/l, 0.36 mmol /l and 13.1 mmol /l for SWV, CV and spectrophotometry respectively. In a conclusion, iron oxide MPs have high pseudo-peroxidase activity resulting in opportunity to use them for the determination of hydrogen peroxide. If an oxidase applied as a biorecognition element, there is no need to co-immobilize peroxidase. Km values were also determined for HRP using spectrophotometry (25.1 mmol/l), SWV (167. mmol/l) and CV (163.2 mmol/l).

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

CB - Analytická chemie, separace

OECD FORD obor

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Projekt

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Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2015

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

International Journal of Electrochemical Science

ISSN

1452-3981

e-ISSN

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Svazek periodika

10

Číslo periodika v rámci svazku

July

Stát vydavatele periodika

CS - Srbsko a Černá Hora

Počet stran výsledku

16

Strana od-do

7033-7048

Kód UT WoS článku

000365101700014

EID výsledku v databázi Scopus

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