Voltammetric biosensor based on a modified chitosan membrane enzyme peroxidase

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60162694%3AG44__%2F16%3A43875672" target="_blank" >RIV/60162694:G44__/16:43875672 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/62156489:43410/16:43910861

Výsledek na webu

<a href="http://www.electrochemsci.org/papers/vol11/111210391.pdf" target="_blank" >http://www.electrochemsci.org/papers/vol11/111210391.pdf</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.20964/2016.12.64" target="_blank" >10.20964/2016.12.64</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Voltammetric biosensor based on a modified chitosan membrane enzyme peroxidase

Popis výsledku v původním jazyce

The determination of hydrogen peroxide (H2O2) has a significant meaning in environmental, pharmaceutical, healthcare and food analyses. The current work is focused on construction of a fast but reliable and accurate voltammetric biosensor enabling detection of H2O2 giving opportunity to its application into practice. This biosensor was based on screen printed electrode covered with chitosan membrane entrapping horseradish peroxidase (HRP), magnetic particles (MPs) and Prussian blue (PB). MPs an inorganic substrate having pseudo-peroxidase activity together with HRP catalyzed reduction of H2O2 and PB as mediator of electron transfer enabled highly sensitive detection at low applied potential. Square wave voltammetry (SWV) was chosen as a detection device. Average area peaks of each H2O2 concentration created calibration curve replying Michaelis-Menten equation with correlation coefficient 0.999. Limit of detection was equal to 8.2 µmol/l of H2O2. Uric acid, ascorbic acid, water soluble derivate of vitamin E (trolox), acetaminophen and reduced glutathione were measured as possibly interfering substances replacing H2O2 in the reaction and no significant influence on the assay was observed when presented in equivalent concentrations as the H2O2. Different personal care matrix substances (tooth paste, hand cream and skin tonic) were spiked with H2O2 and no effect of matrix on the determination was detected. On the basis of the presented results, , the proposed assay was considered to be highly sensitive, accurate and fast assay for detection of H2O2 so this platform can be applied in wide spectrum of research and clinical fields.

Název v anglickém jazyce

Voltammetric biosensor based on a modified chitosan membrane enzyme peroxidase

Popis výsledku anglicky

The determination of hydrogen peroxide (H2O2) has a significant meaning in environmental, pharmaceutical, healthcare and food analyses. The current work is focused on construction of a fast but reliable and accurate voltammetric biosensor enabling detection of H2O2 giving opportunity to its application into practice. This biosensor was based on screen printed electrode covered with chitosan membrane entrapping horseradish peroxidase (HRP), magnetic particles (MPs) and Prussian blue (PB). MPs an inorganic substrate having pseudo-peroxidase activity together with HRP catalyzed reduction of H2O2 and PB as mediator of electron transfer enabled highly sensitive detection at low applied potential. Square wave voltammetry (SWV) was chosen as a detection device. Average area peaks of each H2O2 concentration created calibration curve replying Michaelis-Menten equation with correlation coefficient 0.999. Limit of detection was equal to 8.2 µmol/l of H2O2. Uric acid, ascorbic acid, water soluble derivate of vitamin E (trolox), acetaminophen and reduced glutathione were measured as possibly interfering substances replacing H2O2 in the reaction and no significant influence on the assay was observed when presented in equivalent concentrations as the H2O2. Different personal care matrix substances (tooth paste, hand cream and skin tonic) were spiked with H2O2 and no effect of matrix on the determination was detected. On the basis of the presented results, , the proposed assay was considered to be highly sensitive, accurate and fast assay for detection of H2O2 so this platform can be applied in wide spectrum of research and clinical fields.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

CB - Analytická chemie, separace

OECD FORD obor

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Projekt

—

Návaznosti

S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2016

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

International Journal of Electrochemical Science

ISSN

1452-3981

e-ISSN

—

Svazek periodika

11

Číslo periodika v rámci svazku

12

Stát vydavatele periodika

CS - Srbsko a Černá Hora

Počet stran výsledku

16

Strana od-do

10391-10406

Kód UT WoS článku

000391039000050

EID výsledku v databázi Scopus

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