Piezoelectric Immunosensor for Tissue Transglutaminase Antibodies Determination for Celiac Disease Diagnostic and Comparison with ELISA Method

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60162694%3AG44__%2F20%3A00555984" target="_blank" >RIV/60162694:G44__/20:00555984 - isvavai.cz</a>

Výsledek na webu

<a href="http://www.electrochemsci.org/abstracts/vol15/150605154.pdf" target="_blank" >http://www.electrochemsci.org/abstracts/vol15/150605154.pdf</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.20964/2020.06.36" target="_blank" >10.20964/2020.06.36</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Piezoelectric Immunosensor for Tissue Transglutaminase Antibodies Determination for Celiac Disease Diagnostic and Comparison with ELISA Method

Popis výsledku v původním jazyce

Celiac disease (CD) is a hereditary disease characterized by occurrence of IgA class antibodies against tissue transglutaminase (anti-tTG) in serum. In this work, we compared standard Enzyme-Linked Immunosorbent Assay (ELISA) with a quartz crystal microbalance (QCM) measuring system for determination of anti-tTG. The new immunosensor is based on immobilized tTG in an open conformation (open-tTG) and optimized for assay of IgA anti-tTG with limit of detection 13.3 RU/ml and good specificity and stability over time. Testing serum samples of patients suffering CD showed suitability of the prepared immunosensor for practical performance. Compare to ELISA requiring skilled personnel and expensive equipment, the newly constructed measuring system for measurement of QCM sensor is cheap, easy to prepare and does not require any special skill thus it can be introduced into any laboratory or general practitioner office.

Název v anglickém jazyce

Piezoelectric Immunosensor for Tissue Transglutaminase Antibodies Determination for Celiac Disease Diagnostic and Comparison with ELISA Method

Popis výsledku anglicky

Celiac disease (CD) is a hereditary disease characterized by occurrence of IgA class antibodies against tissue transglutaminase (anti-tTG) in serum. In this work, we compared standard Enzyme-Linked Immunosorbent Assay (ELISA) with a quartz crystal microbalance (QCM) measuring system for determination of anti-tTG. The new immunosensor is based on immobilized tTG in an open conformation (open-tTG) and optimized for assay of IgA anti-tTG with limit of detection 13.3 RU/ml and good specificity and stability over time. Testing serum samples of patients suffering CD showed suitability of the prepared immunosensor for practical performance. Compare to ELISA requiring skilled personnel and expensive equipment, the newly constructed measuring system for measurement of QCM sensor is cheap, easy to prepare and does not require any special skill thus it can be introduced into any laboratory or general practitioner office.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10405 - Electrochemistry (dry cells, batteries, fuel cells, corrosion metals, electrolysis)

Projekt

<a href="/cs/project/TJ01000478" target="_blank" >TJ01000478: Konstrukce senzoru pro kvantitativní stanovení protilátek proti tkáňové transglutaminase pro diagnostiku celiakie</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2020

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

International Journal of Electrochemical Science

ISSN

1452-3981

e-ISSN

—

Svazek periodika

15

Číslo periodika v rámci svazku

6

Stát vydavatele periodika

RS - Srbská republika

Počet stran výsledku

12

Strana od-do

5154-5165

Kód UT WoS článku

000551697600027

EID výsledku v databázi Scopus

2-s2.0-85086838446