Stanovení rezistence bakterií mléčného kvašení ke směsi analogů hořkých chmelových látek tetrahydroizo-alfa-kyselin neinvazivní metodou měření vnitrobuněčného pH

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60193697%3A_____%2F04%3A%230000488" target="_blank" >RIV/60193697:_____/04:#0000488 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Detection of resistance of lactic acid bacteria to a mixture of the hop analogue compounds tetrahydroiso-alpha-acids by noninvasive measurement of intracellular pH

Popis výsledku v původním jazyce

The aim of this article is to examine the resistance of beer isolates of lactic acid bacteria (LAB) towards a mixture of tetrahydroiso-alpha-acids (Tetra) by growth experiments as well as by measurement of intracellular pH (pHi). Beer LAB isolates were identified to species level by SDS-PAGE of whole-cell proteins. Beer isolates of Lactobacillus brevis showed better ability for growth in the presence of Tetra than nonbeer isolates of L.brevis. The antimicrobial effect of Tetra was also examined by noninvasive measurement of pHi by fluorescence ratio imaging microscopy for selected beer isolates of L.brevis and Pediococcus inopinatus. Strains of L. brevis showing limited decrease of pHi during exposure to Tetra also showed better ability of growth in the presence of these compounds as well as in commercial beer products. This study demonstrated the usability of a new rapid method for detecting hop-resistant variants of known beer spoilage LAB species.

Název v anglickém jazyce

Detection of resistance of lactic acid bacteria to a mixture of the hop analogue compounds tetrahydroiso-alpha-acids by noninvasive measurement of intracellular pH

Popis výsledku anglicky

The aim of this article is to examine the resistance of beer isolates of lactic acid bacteria (LAB) towards a mixture of tetrahydroiso-alpha-acids (Tetra) by growth experiments as well as by measurement of intracellular pH (pHi). Beer LAB isolates were identified to species level by SDS-PAGE of whole-cell proteins. Beer isolates of Lactobacillus brevis showed better ability for growth in the presence of Tetra than nonbeer isolates of L.brevis. The antimicrobial effect of Tetra was also examined by noninvasive measurement of pHi by fluorescence ratio imaging microscopy for selected beer isolates of L.brevis and Pediococcus inopinatus. Strains of L. brevis showing limited decrease of pHi during exposure to Tetra also showed better ability of growth in the presence of these compounds as well as in commercial beer products. This study demonstrated the usability of a new rapid method for detecting hop-resistant variants of known beer spoilage LAB species.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EE - Mikrobiologie, virologie

OECD FORD obor

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Projekt

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Návaznosti

N - Vyzkumna aktivita podporovana z neverejnych zdroju

Rok uplatnění

2004

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of Applied Microbiology

ISSN

1364-5072

e-ISSN

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Svazek periodika

96

Číslo periodika v rámci svazku

6

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

9

Strana od-do

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Kód UT WoS článku

000221299700015

EID výsledku v databázi Scopus

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