The determination of deoxynivalenol and zearalenone in barley from Brazil and malted barley

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60193697%3A_____%2F19%3AN0000040" target="_blank" >RIV/60193697:_____/19:N0000040 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

The determination of deoxynivalenol and zearalenone in barley from Brazil and malted barley

Popis výsledku v původním jazyce

The present study describes the determination of mycotoxins deoxynivalenol (DON) and zearalenone (ZEA) in barley from Brazil and in malted barley. The quality of the raw materials involved in the malting process plays a decisive role in the creation of a safe beer product. Immunoaffinity columns for extraction and clean-up, and liquid chromatography coupled with mass spectrometry (LC-MS) are the most commonly used method for determining the presence of mycotoxins. A total of 24 samples were analysed for DON and ZEA. The average concentration of DON and ZEA was 2859.5 μg/kg and 727.9 μg/kg for barley and 1057.3 μg/kg and 275.1 μg/kg for malt, respectively. The content of mycotoxin decreases by about 63% during the malting process. The validation parameters of an analytical method such as linearity, limit of detection (LOD) and limit of quantification (LOQ), and recovery were tested. The calibration curves were linear at least in the range of 10–1000 ng/mL, LOD and LOQ ranging 2.8–3.5 μg/kg and 9.2–11.6 μg/kg, respectively. Recovery for DON and ZEA ranged 87.6%–92.9% with a RSD range of 2.9%–9.2% and 89.6%–105.7% with a RSD range of 0.4%–5.3%, respectively.

Název v anglickém jazyce

The determination of deoxynivalenol and zearalenone in barley from Brazil and malted barley

Popis výsledku anglicky

The present study describes the determination of mycotoxins deoxynivalenol (DON) and zearalenone (ZEA) in barley from Brazil and in malted barley. The quality of the raw materials involved in the malting process plays a decisive role in the creation of a safe beer product. Immunoaffinity columns for extraction and clean-up, and liquid chromatography coupled with mass spectrometry (LC-MS) are the most commonly used method for determining the presence of mycotoxins. A total of 24 samples were analysed for DON and ZEA. The average concentration of DON and ZEA was 2859.5 μg/kg and 727.9 μg/kg for barley and 1057.3 μg/kg and 275.1 μg/kg for malt, respectively. The content of mycotoxin decreases by about 63% during the malting process. The validation parameters of an analytical method such as linearity, limit of detection (LOD) and limit of quantification (LOQ), and recovery were tested. The calibration curves were linear at least in the range of 10–1000 ng/mL, LOD and LOQ ranging 2.8–3.5 μg/kg and 9.2–11.6 μg/kg, respectively. Recovery for DON and ZEA ranged 87.6%–92.9% with a RSD range of 2.9%–9.2% and 89.6%–105.7% with a RSD range of 0.4%–5.3%, respectively.

Druh

D - Stať ve sborníku

CEP obor

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OECD FORD obor

10406 - Analytical chemistry

Projekt

<a href="/cs/project/TE02000177" target="_blank" >TE02000177: Centrum pro inovativní využití a posílení konkurenceschopnosti českých pivovarských surovin a výrobků</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2019

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

MendelNet 2019 Proceedings of 26th International PhD Students Conference

ISBN

978-80-7509-688-3

ISSN

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e-ISSN

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Počet stran výsledku

5

Strana od-do

596-600

Název nakladatele

Mendel University in Brno

Místo vydání

Brno

Místo konání akce

Brno

Datum konání akce

6. 11. 2019

Typ akce podle státní příslušnosti

WRD - Celosvětová akce

Kód UT WoS článku

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