Off-line two-dimensional LC–tandem MS of menaquinones from thermophilic bacteria

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60193697%3A_____%2F24%3AN0000011" target="_blank" >RIV/60193697:_____/24:N0000011 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/60461373:22330/23:43927129 RIV/61388971:_____/24:00579397

Výsledek na webu

<a href="https://www.sciencedirect.com/science/article/pii/S0308814623017302" target="_blank" >https://www.sciencedirect.com/science/article/pii/S0308814623017302</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.plipres.2023.101237" target="_blank" >10.1016/j.plipres.2023.101237</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Off-line two-dimensional LC–tandem MS of menaquinones from thermophilic bacteria

Popis výsledku v původním jazyce

Thermophilic bacteria of four genera in contrast to the commonly used production strains such as Bacillus subtilis, produce homologs other than menaquinone (MK) with seven isoprene units. The number of isoprene units and the configuration of double bonds are essential factors for their biological activity. The goal was to obtain a strain of bacteria that produces a wide range of MK homologs and only all-trans geometrical isomers, which was the strain G. kaustophilus. Using off-line two-dimensional LC–tandem MS in columns with the RP18 phase and the COSMOSIL cholester phase (separation according to the geometric configuration of double bonds) it was shown that thermophilic bacteria grown at different temperatures produce only all-trans isomers of menaquinones from MK-5 (menaquinone with five isoprenyl units) to MK-15 (fifteen isoprenyl units). Therefore, G. kaustophilus appears to be a biotechnologically important strain produces only trans isomers and additionally homologs from 5 to 15 isoprene units.

Název v anglickém jazyce

Off-line two-dimensional LC–tandem MS of menaquinones from thermophilic bacteria

Popis výsledku anglicky

Thermophilic bacteria of four genera in contrast to the commonly used production strains such as Bacillus subtilis, produce homologs other than menaquinone (MK) with seven isoprene units. The number of isoprene units and the configuration of double bonds are essential factors for their biological activity. The goal was to obtain a strain of bacteria that produces a wide range of MK homologs and only all-trans geometrical isomers, which was the strain G. kaustophilus. Using off-line two-dimensional LC–tandem MS in columns with the RP18 phase and the COSMOSIL cholester phase (separation according to the geometric configuration of double bonds) it was shown that thermophilic bacteria grown at different temperatures produce only all-trans isomers of menaquinones from MK-5 (menaquinone with five isoprenyl units) to MK-15 (fifteen isoprenyl units). Therefore, G. kaustophilus appears to be a biotechnologically important strain produces only trans isomers and additionally homologs from 5 to 15 isoprene units.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10606 - Microbiology

Projekt

—

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2024

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Food Chemistry

ISSN

0308-8146

e-ISSN

1873-7072

Svazek periodika

431

Číslo periodika v rámci svazku

15 January 2024

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

8

Strana od-do

137112

Kód UT WoS článku

001055227900001

EID výsledku v databázi Scopus

2-s2.0-85167800427