Oryzalin induces polyploids with superior morphology and increased levels of essential oil production in Mentha spicata L

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60460709%3A41110%2F23%3A94619" target="_blank" >RIV/60460709:41110/23:94619 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/60460709:41210/23:94619 RIV/60460709:41340/23:94619

Výsledek na webu

<a href="https://www.webofscience.com/wos/woscc/full-record/WOS:000982489400001" target="_blank" >https://www.webofscience.com/wos/woscc/full-record/WOS:000982489400001</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.indcrop.2023.116683" target="_blank" >10.1016/j.indcrop.2023.116683</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Oryzalin induces polyploids with superior morphology and increased levels of essential oil production in Mentha spicata L

Popis výsledku v původním jazyce

Efficacy of oryzalin to generate novel genotypes of Mentha spicata L. with superior morphology and enhanced levels of essential oil via in vitro polyploidization was assessed. Micropropagated nodal segments were treated with oryzalin at 20, 40, and 60 mu M for 24 and 48 h. The survival rate of the nodal segments dropped significantly with the increasing concentration and duration of oryzalin. Further, the ploidy level of the survived plants was confirmed using flowcytometry and chromosome counting. A total of six polyploids (2 n = 6x = 72) and six mixoploid plants were obtained. The highest frequency of polyploids (8 %) was induced in treatment where 40 mu M oryzalin was applied for 48 h. The obtained polyploids exhibited significantly larger, thick, dark green leaves with elongated stomata compared with the control genotype. The polyploid plants also displayed higher moisture content and vigorous lateral growth. The essential oil yield of hexaploid M. spicata exhibited a 48,85 % increase, rising from 1,74 % in control to 2,59 % (v/w) in genotype P3. The major components of essential oils were carvone and limonene, which also increased significantly compared with the control genotype. The nutrient profile of the studied genotypes was influenced vastly and showed significant variations. Overall, the employed methodology using oryzalin as an antimitotic agent for polyploid induction in M. spicata was found to be effective. Despite relatively low polyploid induction frequency, the obtained polyploid genotypes demonstrated various superior agronomical traits. The genotypes obtained could serve to aid the commercial demands.

Název v anglickém jazyce

Oryzalin induces polyploids with superior morphology and increased levels of essential oil production in Mentha spicata L

Popis výsledku anglicky

Efficacy of oryzalin to generate novel genotypes of Mentha spicata L. with superior morphology and enhanced levels of essential oil via in vitro polyploidization was assessed. Micropropagated nodal segments were treated with oryzalin at 20, 40, and 60 mu M for 24 and 48 h. The survival rate of the nodal segments dropped significantly with the increasing concentration and duration of oryzalin. Further, the ploidy level of the survived plants was confirmed using flowcytometry and chromosome counting. A total of six polyploids (2 n = 6x = 72) and six mixoploid plants were obtained. The highest frequency of polyploids (8 %) was induced in treatment where 40 mu M oryzalin was applied for 48 h. The obtained polyploids exhibited significantly larger, thick, dark green leaves with elongated stomata compared with the control genotype. The polyploid plants also displayed higher moisture content and vigorous lateral growth. The essential oil yield of hexaploid M. spicata exhibited a 48,85 % increase, rising from 1,74 % in control to 2,59 % (v/w) in genotype P3. The major components of essential oils were carvone and limonene, which also increased significantly compared with the control genotype. The nutrient profile of the studied genotypes was influenced vastly and showed significant variations. Overall, the employed methodology using oryzalin as an antimitotic agent for polyploid induction in M. spicata was found to be effective. Despite relatively low polyploid induction frequency, the obtained polyploid genotypes demonstrated various superior agronomical traits. The genotypes obtained could serve to aid the commercial demands.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

40101 - Agriculture

Projekt

—

Návaznosti

S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2023

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

INDUSTRIAL CROPS AND PRODUCTS

ISSN

0926-6690

e-ISSN

0926-6690

Svazek periodika

198

Číslo periodika v rámci svazku

Aug 2023

Stát vydavatele periodika

CZ - Česká republika

Počet stran výsledku

10

Strana od-do

—

Kód UT WoS článku

000982489400001

EID výsledku v databázi Scopus

2-s2.0-85151806021