Artificial induction of tetraploidy in Humulus lupulus L. using oryzalin

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15310%2F19%3A73596541" target="_blank" >RIV/61989592:15310/19:73596541 - isvavai.cz</a>

Výsledek na webu

<a href="https://pbsociety.org.pl/journals/index.php/aa/article/view/aa.1764/7718" target="_blank" >https://pbsociety.org.pl/journals/index.php/aa/article/view/aa.1764/7718</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.5586/aa.1764" target="_blank" >10.5586/aa.1764</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Artificial induction of tetraploidy in Humulus lupulus L. using oryzalin

Popis výsledku v původním jazyce

The aim of the research was to establish an efficient procedure for in vitro micropropagation in order to obtain numbers of identical plants for in vitro polyploidization of Humulus lupulus (2n = 20), using antimicrotubule agent oryzalin. For this purpose, the polyploidization was carried out for H. lupulus Osvald&apos;s clones 31, 74, 114, and for &apos;Sladek&apos; cultivar. The two experimental methods - the cultivation of nodal segments on medium with different concentrations of oryzalin (1, 5, and 10 mu M) for 2 weeks and the irrigation of nodal segments with oryzalin (10 and 20 mu M) for 24 and 48 h were chosen for inducing for polyploid plantlets of H. lupulus. This procedure provided tetraploids, which were identified by flow cytometry using internal standardization method and confirmed using chromosome counting of methaphasic cells from the root tips and morphological observations. The influence of chromosome doubling was also verified with stomata characterization. The polyploid plants were propagated for next evaluation, rooting and transfer to nonsterile conditions and into field. After chromosome doubling, using some different concentration of oryzalin, some plantlets became tetraploids, no mixoploids were detected. The highest efficiency of polyploidization was achieved for clone 72 after 2-week treatment of oryzalin supplemented medium. On the other hand, for method based on the irrigation of nodal segments with oryzalin, the most efficient conditions were treatment with 10 mu M and 20 mu M oryzalin for 24 and 48 h, respectively.

Název v anglickém jazyce

Artificial induction of tetraploidy in Humulus lupulus L. using oryzalin

Popis výsledku anglicky

The aim of the research was to establish an efficient procedure for in vitro micropropagation in order to obtain numbers of identical plants for in vitro polyploidization of Humulus lupulus (2n = 20), using antimicrotubule agent oryzalin. For this purpose, the polyploidization was carried out for H. lupulus Osvald&apos;s clones 31, 74, 114, and for &apos;Sladek&apos; cultivar. The two experimental methods - the cultivation of nodal segments on medium with different concentrations of oryzalin (1, 5, and 10 mu M) for 2 weeks and the irrigation of nodal segments with oryzalin (10 and 20 mu M) for 24 and 48 h were chosen for inducing for polyploid plantlets of H. lupulus. This procedure provided tetraploids, which were identified by flow cytometry using internal standardization method and confirmed using chromosome counting of methaphasic cells from the root tips and morphological observations. The influence of chromosome doubling was also verified with stomata characterization. The polyploid plants were propagated for next evaluation, rooting and transfer to nonsterile conditions and into field. After chromosome doubling, using some different concentration of oryzalin, some plantlets became tetraploids, no mixoploids were detected. The highest efficiency of polyploidization was achieved for clone 72 after 2-week treatment of oryzalin supplemented medium. On the other hand, for method based on the irrigation of nodal segments with oryzalin, the most efficient conditions were treatment with 10 mu M and 20 mu M oryzalin for 24 and 48 h, respectively.

Druh

J_SC - Článek v periodiku v databázi SCOPUS

CEP obor

—

OECD FORD obor

40101 - Agriculture

Projekt

<a href="/cs/project/QJ1510160" target="_blank" >QJ1510160: Nové technologie získávání biologicky aktivních látek z léčivých a aromatických rostlin jako zdrojů účinných látek botanických pesticidů a potravinových doplňků</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2019

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

ACTA AGROBOTANICA

ISSN

0065-0951

e-ISSN

—

Svazek periodika

72

Číslo periodika v rámci svazku

2

Stát vydavatele periodika

PL - Polská republika

Počet stran výsledku

10

Strana od-do

"1764-1"-"1764-10"

Kód UT WoS článku

000474433100001

EID výsledku v databázi Scopus

2-s2.0-85068898652