Induced polyploidization and its influence on yield, morphological, and qualitative characteristics of microtubers in Ullucus tuberosus

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F70883521%3A28110%2F12%3A43867822" target="_blank" >RIV/70883521:28110/12:43867822 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/67985939:_____/12:00381125 RIV/60460709:41610/12:54461 RIV/00216208:11310/12:10196802

Výsledek na webu

<a href="http://dx.doi.org/10.1007/s11240-011-0076-7" target="_blank" >http://dx.doi.org/10.1007/s11240-011-0076-7</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s11240-011-0076-7" target="_blank" >10.1007/s11240-011-0076-7</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Induced polyploidization and its influence on yield, morphological, and qualitative characteristics of microtubers in Ullucus tuberosus

Popis výsledku v původním jazyce

This study aimed to induce in vitro polyploidization in diploid ulluco (2n = 24) using oryzalin, an antimicrotubule agent. Nodal segments were treated with 0, 20, 25 or 30 mu M oryzalin for 24 or 48 h. Ploidy determination of regenerants was based on flow cytometry and chromosome countings in metaphasic cells from root tips. After polyploidization, microtuber formation was induced in plantlets. Determination of starch content using spectrophotometric analysis, and vitamin C content by titration with 2,6-dichlorphenolindophenol were carried out to assess diploid and octoploid microtubers. Colour of in vitro plantlets was evaluated using the RHS colour chart. Out of 120 nodal segments exposed to oryzalin, 2.5% were octoploids (2n = 96). The most efficient conditions for inducing polyploidy were treatment with 20 mu M oryzalin for 24 h, followed by 25 mu M oryzalin for 48 h. No tetraploids were obtained. Moreover, flow cytometric analyses of the nuclear DNA contents revealed extensive end

Název v anglickém jazyce

Induced polyploidization and its influence on yield, morphological, and qualitative characteristics of microtubers in Ullucus tuberosus

Popis výsledku anglicky

This study aimed to induce in vitro polyploidization in diploid ulluco (2n = 24) using oryzalin, an antimicrotubule agent. Nodal segments were treated with 0, 20, 25 or 30 mu M oryzalin for 24 or 48 h. Ploidy determination of regenerants was based on flow cytometry and chromosome countings in metaphasic cells from root tips. After polyploidization, microtuber formation was induced in plantlets. Determination of starch content using spectrophotometric analysis, and vitamin C content by titration with 2,6-dichlorphenolindophenol were carried out to assess diploid and octoploid microtubers. Colour of in vitro plantlets was evaluated using the RHS colour chart. Out of 120 nodal segments exposed to oryzalin, 2.5% were octoploids (2n = 96). The most efficient conditions for inducing polyploidy were treatment with 20 mu M oryzalin for 24 h, followed by 25 mu M oryzalin for 48 h. No tetraploids were obtained. Moreover, flow cytometric analyses of the nuclear DNA contents revealed extensive end

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

GM - Potravinářství

OECD FORD obor

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Projekt

—

Návaznosti

V - Vyzkumna aktivita podporovana z jinych verejnych zdroju

Rok uplatnění

2012

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Plant Cell, Tissue and Organ Culture: an international journal on in vitro culture of higher plants

ISSN

0167-6857

e-ISSN

—

Svazek periodika

109

Číslo periodika v rámci svazku

1

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

8

Strana od-do

83-90

Kód UT WoS článku

000301599800009

EID výsledku v databázi Scopus

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