Molecular Markers of Apple Trees Resistance against Scab (Venturia inaequalis CKE.)

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60460709%3A41210%2F01%3A00002909" target="_blank" >RIV/60460709:41210/01:00002909 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Molecular Markers of Apple Trees Resistance against Scab (Venturia inaequalis CKE.)

Popis výsledku v původním jazyce

A set of dominant and co-dominant PCR markers was used to detect the Vf alleles. This methodological procedure was verified on a collection of apple tree varieties with a declared resistance against scab, determined by the dominant Vf gene. Nine reference varieties containing the Vf gene were used, developed by the Research and Breeding Institute of Pomology in Holovousy. Conducted was also a segregation analysis of seedlings of eight complex combination crossbreeds. The resistance or sensitivity of theassessed plant material was also classified in standard greenhouse infection tests. The so-called multi-PCR was used to detect alleles of the Vf gene. The actual amplification and sequences of primer pairs were obtained from Tartarini et al. (1999) exp eriments. The multi-PCR used allowed a simultaneous amplification of one dominant and one co-dominant marker. The co-dominant marker enabled the dominant homozygotes, heterozygotes and recessive homozygotes to be distinguished from each ot

Název v anglickém jazyce

Molecular Markers of Apple Trees Resistance against Scab (Venturia inaequalis CKE.)

Popis výsledku anglicky

A set of dominant and co-dominant PCR markers was used to detect the Vf alleles. This methodological procedure was verified on a collection of apple tree varieties with a declared resistance against scab, determined by the dominant Vf gene. Nine reference varieties containing the Vf gene were used, developed by the Research and Breeding Institute of Pomology in Holovousy. Conducted was also a segregation analysis of seedlings of eight complex combination crossbreeds. The resistance or sensitivity of theassessed plant material was also classified in standard greenhouse infection tests. The so-called multi-PCR was used to detect alleles of the Vf gene. The actual amplification and sequences of primer pairs were obtained from Tartarini et al. (1999) exp eriments. The multi-PCR used allowed a simultaneous amplification of one dominant and one co-dominant marker. The co-dominant marker enabled the dominant homozygotes, heterozygotes and recessive homozygotes to be distinguished from each ot

Druh

D - Stať ve sborníku

CEP obor

GE - Šlechtění rostlin

OECD FORD obor

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Projekt

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Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2001

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

4th International Symposium in the Series Recent Advances in Plant Biotechnology, Plant Molecular Biology for the New Millennium, Book of Abstracts

ISBN

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ISSN

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e-ISSN

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Počet stran výsledku

1

Strana od-do

49

Název nakladatele

Ústav molekulární biologie rostlin AV ČR

Místo vydání

České Budějovice

Místo konání akce

Třeboň

Datum konání akce

1. 1. 2001

Typ akce podle státní příslušnosti

EUR - Evropská akce

Kód UT WoS článku

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