Changes of proteins induced by arrested development (hypobiosis) in Trichostrongylus colubriformis

Kód výsledku v IS VaVaI

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Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Changes of proteins induced by arrested development (hypobiosis) in Trichostrongylus colubriformis

Popis výsledku v původním jazyce

The infective larvae were induced by hypobiosis triggering factors which included low temperature (5°C) and decreasing photoperiods, for 5 weeks. The appearance of variatons in the protein profile of Trichostrongylus colubriformis (Giles, 1892) was evaluated by means of SDS-PAGE and densitometric analysis. Important quantitative variations were identified in the protein levels of the induced larvae, where the amount of two polypeptides (molecular weight 200-220 kDa) decreased in range 32,3-35,4% and theamount of six polypeptides (molecular weight 20-28 kDa) increased in range 20,0-27,0% compared to the control sample. Control of hypobiosis was effected by inoculation to two groups of gerbils (Meriones unguiculatus). The first group was infected by co nditoned larvae and the second group was control. Rate of inhibited larvae of group with conditioned larvae was in range 55-100%, in control group with unconditioned larvae was rate of inhibited larvae 0 - 14%. This work demonstrated chang

Název v anglickém jazyce

Changes of proteins induced by arrested development (hypobiosis) in Trichostrongylus colubriformis

Popis výsledku anglicky

The infective larvae were induced by hypobiosis triggering factors which included low temperature (5°C) and decreasing photoperiods, for 5 weeks. The appearance of variatons in the protein profile of Trichostrongylus colubriformis (Giles, 1892) was evaluated by means of SDS-PAGE and densitometric analysis. Important quantitative variations were identified in the protein levels of the induced larvae, where the amount of two polypeptides (molecular weight 200-220 kDa) decreased in range 32,3-35,4% and theamount of six polypeptides (molecular weight 20-28 kDa) increased in range 20,0-27,0% compared to the control sample. Control of hypobiosis was effected by inoculation to two groups of gerbils (Meriones unguiculatus). The first group was infected by co nditoned larvae and the second group was control. Rate of inhibited larvae of group with conditioned larvae was in range 55-100%, in control group with unconditioned larvae was rate of inhibited larvae 0 - 14%. This work demonstrated chang

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EG - Zoologie

OECD FORD obor

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Projekt

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Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2002

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Helmintologia

ISSN

0440-6605

e-ISSN

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Svazek periodika

39

Číslo periodika v rámci svazku

3

Stát vydavatele periodika

SK - Slovenská republika

Počet stran výsledku

1

Strana od-do

171

Kód UT WoS článku

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EID výsledku v databázi Scopus

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