Enzymatické a proteinové změny mezi infekčními larvami richostrongylus colubriformis indukovanými a neindukovanými k hypobióze

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60460709%3A41210%2F06%3A16318" target="_blank" >RIV/60460709:41210/06:16318 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/67985823:_____/06:00044605

Výsledek na webu

—

DOI - Digital Object Identifier

—

Jazyk výsledku

angličtina

Název v původním jazyce

Enzymatic and protein differences between infective larvae of Trichostrongylus colubriformis conditioned or not conditioned to hypobiosis

Popis výsledku v původním jazyce

The differences in protein and enzymatic profiles of infective larvae (L3) of Trichostrongylus colubriformis, both induced and non-induced to hypobiosis, have been evaluated by means of SDS-PAGE and densitometric analysis as well as by semiquantitative micromethod API-ZYM (Bio- Mérieux, France). Quantitative differences were identified in protein levels between the induced and non-induced larvae, where the amount of two polypeptides (200 ? 220 kDa) decreased in range 32.3 ? 35.4 % and the amount of sixpolypeptides (20 ? 28 kDa) increased in range 20.0 ? 27.0 % in the samples of induced larvae. In contrast to non-induced larvae, on gelatin-substrate gel in L3 in vitro released (IVR) proteases from larvae conditioned to hypobiosis, zones of proteolysiswere observed between 21 and 34 kDa.

Název v anglickém jazyce

Enzymatic and protein differences between infective larvae of Trichostrongylus colubriformis conditioned or not conditioned to hypobiosis

Popis výsledku anglicky

The differences in protein and enzymatic profiles of infective larvae (L3) of Trichostrongylus colubriformis, both induced and non-induced to hypobiosis, have been evaluated by means of SDS-PAGE and densitometric analysis as well as by semiquantitative micromethod API-ZYM (Bio- Mérieux, France). Quantitative differences were identified in protein levels between the induced and non-induced larvae, where the amount of two polypeptides (200 ? 220 kDa) decreased in range 32.3 ? 35.4 % and the amount of sixpolypeptides (20 ? 28 kDa) increased in range 20.0 ? 27.0 % in the samples of induced larvae. In contrast to non-induced larvae, on gelatin-substrate gel in L3 in vitro released (IVR) proteases from larvae conditioned to hypobiosis, zones of proteolysiswere observed between 21 and 34 kDa.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

GJ - Choroby a škůdci zvířat, veterinární medicina

OECD FORD obor

—

Projekt

—

Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2006

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Helmintologia

ISSN

0440-6605

e-ISSN

—

Svazek periodika

43

Číslo periodika v rámci svazku

2

Stát vydavatele periodika

SK - Slovenská republika

Počet stran výsledku

5

Strana od-do

64-68

Kód UT WoS článku

—

EID výsledku v databázi Scopus

—