DETECTION OF HOP STUNT VIROID WITHIN POLLEN GRAINS OF INFECTED PEACH TREES: EVIDENCE USING RT-PCR AND DOT BLOT HYBRIDIZATION

Kód výsledku v IS VaVaI

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Nalezeny alternativní kódy

RIV/60460709:_____/03:00005226

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

DETECTION OF HOP STUNT VIROID WITHIN POLLEN GRAINS OF INFECTED PEACH TREES: EVIDENCE USING RT-PCR AND DOT BLOT HYBRIDIZATION

Popis výsledku v původním jazyce

Hop stunt viroid (HSVd) is the viroid with the broadest host range known so far, also including stone fruit trees. Until now, six viroids have been shown to be transmitted through seeds to seedlings, in one or more hosts, four of them have been detectedin pollen, cucumber strain of HSVd being among them. High incidence of HSVd infections of peach trees was recently found in the Czech Republic indicating the possibility of spreading via pollen. That is why we tested the presence of HSVd in pollen grains. Closed flowers from peach trees of cultivars Sunhaven and NJC 103 known to be infected by HSVd were collected in March. Pollen grains were separated from air dried anthers and total RNA was extracted from 10 - 20 mg of pollen or intact anthers and from100 mg of leaves or petals. PCR and molecular hybridization methods were used for HSVd detection. HSVd was consistently detected in pollen, anthers, petals as well as in leaves in each of at least three repetitions. This preliminary resul

Název v anglickém jazyce

DETECTION OF HOP STUNT VIROID WITHIN POLLEN GRAINS OF INFECTED PEACH TREES: EVIDENCE USING RT-PCR AND DOT BLOT HYBRIDIZATION

Popis výsledku anglicky

Hop stunt viroid (HSVd) is the viroid with the broadest host range known so far, also including stone fruit trees. Until now, six viroids have been shown to be transmitted through seeds to seedlings, in one or more hosts, four of them have been detectedin pollen, cucumber strain of HSVd being among them. High incidence of HSVd infections of peach trees was recently found in the Czech Republic indicating the possibility of spreading via pollen. That is why we tested the presence of HSVd in pollen grains. Closed flowers from peach trees of cultivars Sunhaven and NJC 103 known to be infected by HSVd were collected in March. Pollen grains were separated from air dried anthers and total RNA was extracted from 10 - 20 mg of pollen or intact anthers and from100 mg of leaves or petals. PCR and molecular hybridization methods were used for HSVd detection. HSVd was consistently detected in pollen, anthers, petals as well as in leaves in each of at least three repetitions. This preliminary resul

Druh

D - Stať ve sborníku

CEP obor

GF - Choroby, škůdci, plevely a ochrana rostlin

OECD FORD obor

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Projekt

<a href="/cs/project/QC1359" target="_blank" >QC1359: Výzkum a vývoj komplexních postupů diagnostiky hospodářsky významných a karanténních fytopatogenních organismů pro systém certifikace ovocných dřevin.</a><br>

Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2003

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

Book of abstract 19th Int. Symp. Virus and Virus - like Diseases of Temperature Fruit Crops

ISBN

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ISSN

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e-ISSN

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Počet stran výsledku

1

Strana od-do

117

Název nakladatele

ISHS

Místo vydání

Valencia

Místo konání akce

Valencia

Datum konání akce

21. 7. 2003

Typ akce podle státní příslušnosti

WRD - Celosvětová akce

Kód UT WoS článku

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