Vývoj metody RT-PCR pro detekci viroidu latentní mozaiky broskvoně a viroidu zakrslosti chmele pro účely certifikace sadbového materiálu

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60460709%3A41210%2F05%3A%23%23%2312265" target="_blank" >RIV/60460709:41210/05:###12265 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Development of a PCR method of Peach latent mosaic viroid and Hop stunt viroid detection for certification of planting material.

Popis výsledku v původním jazyce

High incidence of Peach latent mosaic viroid (PLMVd) and Hop stunt viroid (HSVd) infections of peach trees was recently found in the Czech Republic. In the frame of prepared certification system for fruit trees planting material a PCR method for these purposes had to be developed. Several primer pairs were designed according to the published sequences of both viroids. Various plant material (leaves, flowers, pollen, bark) was tested during the year and also several methods of RNA extraction were tested.PCR with different primer pairs gives reliable results. More important is the time of testing, material, and RNA extraction method. The best period for detection of both viroids is late spring and early summer taking leaves as testing material and usingany of RNA extraction methods. For older leaves in late summer using of Qiagen kit for RNA extraction can be recommended. Detection of both viroids in bark is possible even during winter but it is not very reliable. Only HSVd can be detect

Název v anglickém jazyce

Development of a PCR method of Peach latent mosaic viroid and Hop stunt viroid detection for certification of planting material.

Popis výsledku anglicky

High incidence of Peach latent mosaic viroid (PLMVd) and Hop stunt viroid (HSVd) infections of peach trees was recently found in the Czech Republic. In the frame of prepared certification system for fruit trees planting material a PCR method for these purposes had to be developed. Several primer pairs were designed according to the published sequences of both viroids. Various plant material (leaves, flowers, pollen, bark) was tested during the year and also several methods of RNA extraction were tested.PCR with different primer pairs gives reliable results. More important is the time of testing, material, and RNA extraction method. The best period for detection of both viroids is late spring and early summer taking leaves as testing material and usingany of RNA extraction methods. For older leaves in late summer using of Qiagen kit for RNA extraction can be recommended. Detection of both viroids in bark is possible even during winter but it is not very reliable. Only HSVd can be detect

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

GF - Choroby, škůdci, plevely a ochrana rostlin

OECD FORD obor

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Projekt

<a href="/cs/project/QC1359" target="_blank" >QC1359: Výzkum a vývoj komplexních postupů diagnostiky hospodářsky významných a karanténních fytopatogenních organismů pro systém certifikace ovocných dřevin.</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2005

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Acta Horticulturae

ISSN

0567-7572

e-ISSN

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Svazek periodika

5

Číslo periodika v rámci svazku

657

Stát vydavatele periodika

BE - Belgické království

Počet stran výsledku

5

Strana od-do

391-395

Kód UT WoS článku

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EID výsledku v databázi Scopus

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