DEVELOPMENT OF PCR METHOD OF PEACH LATENT MOSAIC VIROID AND HOP STUNT VIROID DETECTION FOR CERTIFICATION OF PLANTING MATERIAL

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60460709%3A41210%2F03%3A00005230" target="_blank" >RIV/60460709:41210/03:00005230 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/60460709:_____/03:00005230

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

DEVELOPMENT OF PCR METHOD OF PEACH LATENT MOSAIC VIROID AND HOP STUNT VIROID DETECTION FOR CERTIFICATION OF PLANTING MATERIAL

Popis výsledku v původním jazyce

High incidence of Peach latent mosaic viroid (PLMVd) and Hop stunt viroid (HSVd) infections of peach trees was recently found in the Czech Republic. In the frame of prepared certification system for fruit trees planting material a PCR method for these purposes had to be developed. Several primer pairs were designed according to the published sequences of both viroids. Various plant material (leaves, flowers, pollen, bark) was tested during the year and also several methods of RNA extraction were tested.PCR with different primer pairs gives reliable results. More important is the time of testing, material, and RNA extraction method. The best period for detection of both viroids is late spring and early summer taking leaves as testing material and usin gany of RNA extraction methods. For older leaves in late summer using of Qiagen kit for RNA extraction can be recommended. Detection of both viroids in bark is possible even during winter but it is not very reliable. Only HSVd can be dete

Název v anglickém jazyce

DEVELOPMENT OF PCR METHOD OF PEACH LATENT MOSAIC VIROID AND HOP STUNT VIROID DETECTION FOR CERTIFICATION OF PLANTING MATERIAL

Popis výsledku anglicky

High incidence of Peach latent mosaic viroid (PLMVd) and Hop stunt viroid (HSVd) infections of peach trees was recently found in the Czech Republic. In the frame of prepared certification system for fruit trees planting material a PCR method for these purposes had to be developed. Several primer pairs were designed according to the published sequences of both viroids. Various plant material (leaves, flowers, pollen, bark) was tested during the year and also several methods of RNA extraction were tested.PCR with different primer pairs gives reliable results. More important is the time of testing, material, and RNA extraction method. The best period for detection of both viroids is late spring and early summer taking leaves as testing material and usin gany of RNA extraction methods. For older leaves in late summer using of Qiagen kit for RNA extraction can be recommended. Detection of both viroids in bark is possible even during winter but it is not very reliable. Only HSVd can be dete

Druh

D - Stať ve sborníku

CEP obor

GF - Choroby, škůdci, plevely a ochrana rostlin

OECD FORD obor

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Projekt

<a href="/cs/project/QC1359" target="_blank" >QC1359: Výzkum a vývoj komplexních postupů diagnostiky hospodářsky významných a karanténních fytopatogenních organismů pro systém certifikace ovocných dřevin.</a><br>

Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2003

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

Book of abstract 19th Int. Symp. Virus and Virus - like Diseases of Temperature Fruit Crops

ISBN

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ISSN

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e-ISSN

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Počet stran výsledku

1

Strana od-do

115

Název nakladatele

ISHS

Místo vydání

Valencia

Místo konání akce

Valencia

Datum konání akce

21. 7. 2003

Typ akce podle státní příslušnosti

WRD - Celosvětová akce

Kód UT WoS článku

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