Účinek inhibitoru proteazómu MG132 na meiotické zrání prasečích oocytů

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60460709%3A41210%2F04%3A7180" target="_blank" >RIV/60460709:41210/04:7180 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Effect of proteasome inhibitor MG132 on in vitro maturation of porcine oocytes

Popis výsledku v původním jazyce

The study aimed to demonstrate the dependence of meiotic maturation in pig oocytes on the activity of the protease complex proteasome. The proteasome inhibitor MG132blocked the exit of maturing pig oocytes from I metaphase stage. 75% of the oocytes wereblocked at MI stage when they were cultured with 10 M MG132. The blocking effect of MG132 was expressed only when the oocytes were exposed an inhibitor before the 18th hour of culture. The effects of MG132 are fully reversible. However a significant proportion of oocytes (46%) cultured for 48 hours in MG132 supplemented medium and then for 24 h in MG132-free medium did not block meiosis at the stage of metaphase II and underwent spontaneous parthenogenetic activation. On the basis of our data we can conclude that exit from the metaphase I stage of meiosis is preoteasome-dependent in pig oocytes matured in vitro. On the other hand, our data also indicate that other proteasome-independent events are involved in regulating the exit from me

Název v anglickém jazyce

Effect of proteasome inhibitor MG132 on in vitro maturation of porcine oocytes

Popis výsledku anglicky

The study aimed to demonstrate the dependence of meiotic maturation in pig oocytes on the activity of the protease complex proteasome. The proteasome inhibitor MG132blocked the exit of maturing pig oocytes from I metaphase stage. 75% of the oocytes wereblocked at MI stage when they were cultured with 10 M MG132. The blocking effect of MG132 was expressed only when the oocytes were exposed an inhibitor before the 18th hour of culture. The effects of MG132 are fully reversible. However a significant proportion of oocytes (46%) cultured for 48 hours in MG132 supplemented medium and then for 24 h in MG132-free medium did not block meiosis at the stage of metaphase II and underwent spontaneous parthenogenetic activation. On the basis of our data we can conclude that exit from the metaphase I stage of meiosis is preoteasome-dependent in pig oocytes matured in vitro. On the other hand, our data also indicate that other proteasome-independent events are involved in regulating the exit from me

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

GG - Chov hospodářských zvířat

OECD FORD obor

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Projekt

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Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2004

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Zygote

ISSN

0967-1994

e-ISSN

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Svazek periodika

12

Číslo periodika v rámci svazku

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Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

6

Strana od-do

157-162

Kód UT WoS článku

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EID výsledku v databázi Scopus

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