Histone deacetylase inhibition improves meiotic competence but not developmental competence in growing pig oocytes

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027014%3A_____%2F09%3A%230001104" target="_blank" >RIV/00027014:_____/09:#0001104 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Histone deacetylase inhibition improves meiotic competence but not developmental competence in growing pig oocytes

Popis výsledku v původním jazyce

In fully grown pig oocytes, meiotic maturation in vitro is slowed by inhibition of histone deacetylases by trichostatin A (TSA). In growing oocytes with partial meiotic competence, the culture with TSA has no significant effect on the meitic maturation.Growing oocytes treated with TSA mature mainly to the stage of metaphase I. The ratio of oocytes maturing to the stage of metaphase II is very limited. After the transient exposure to TSA, the maturation of growing oocytes with partial meiotic competencetakes another course. When these oocytes are first cultured in TSA-free medium, then cultured for another 24 hours with 100 nM TSA and finally they are again cultured in TSA-free medium for 24 hours, the ratio of oocytes maturing to the stage of metaphase II is significantly increased and it reached 59%. When oocytes are cultured for the same time without transient exposure to TSA, only 19% matured to the stage of metaphase II.

Název v anglickém jazyce

Histone deacetylase inhibition improves meiotic competence but not developmental competence in growing pig oocytes

Popis výsledku anglicky

In fully grown pig oocytes, meiotic maturation in vitro is slowed by inhibition of histone deacetylases by trichostatin A (TSA). In growing oocytes with partial meiotic competence, the culture with TSA has no significant effect on the meitic maturation.Growing oocytes treated with TSA mature mainly to the stage of metaphase I. The ratio of oocytes maturing to the stage of metaphase II is very limited. After the transient exposure to TSA, the maturation of growing oocytes with partial meiotic competencetakes another course. When these oocytes are first cultured in TSA-free medium, then cultured for another 24 hours with 100 nM TSA and finally they are again cultured in TSA-free medium for 24 hours, the ratio of oocytes maturing to the stage of metaphase II is significantly increased and it reached 59%. When oocytes are cultured for the same time without transient exposure to TSA, only 19% matured to the stage of metaphase II.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EB - Genetika a molekulární biologie

OECD FORD obor

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Projekt

<a href="/cs/project/QG50052" target="_blank" >QG50052: Reprodukční biotechnologie u prasat</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2009

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Zygote

ISSN

0967-1994

e-ISSN

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Svazek periodika

17

Číslo periodika v rámci svazku

4

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

8

Strana od-do

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Kód UT WoS článku

000271184000004

EID výsledku v databázi Scopus

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